resumo
Herein, the effective use of aqueous biphasic systems (ABS) in Fast Centrifugal Partition Chromatography (FCPC) for the purification of PEGylated cytochrome c conjugates is shown. High recoveries (between 88% and 100%) and purities (similar to 100%) were obtained. Both the unreacted cytochrome c and solvents may be recovered and reused, thus allowing the design of a sustainable process in a continuous regime for the isolation of bioconjugates. This process allowed the reduction of the complete E-factor and carbon footprint at circa 100% and 67%, respectively, reinforcing the important environmental contribution of recycling units.
palavras-chave
AQUEOUS 2-PHASE SYSTEMS; EXTRUSION COUNTERCURRENT CHROMATOGRAPHY; POLYETHYLENE-GLYCOL; L-ASPARAGINASE; CHEMISTRY; PHASE; FRACTIONATION; PURIFICATION; PROTEINS; RESIDUES
categoria
Chemistry; Science & Technology - Other Topics
autores
Santos, JHPM; Ferreira, AM; Almeida, MR; Quinteiro, PSGN; Dias, ACRV; Coutinho, JAP; Freire, MG; Rangel-Yagui, CO; Ventura, SPM
nossos autores
Ana Mafalda Rodrigues Almeida Rocha
Investigador
Ana Maria da Conceição Ferreira Takahashi
Investigador Júnior
João Henrique Picado Madalena Santos
Estudante de doutoramento
Mara Guadalupe Freire Martins
Investigador Coordenador
Sónia Patrícia Marques Ventura
Professor AuxiliarGrupos
G4 - Materiais Renováveis e Economia Circular
G5 - Materiais Biomiméticos, Biológicos e Vivos
Projectos
CICECO - Aveiro Institute of Materials (UID/CTM/50011/2019)
Projeto de Investigação Exploratória: Sónia Patricia Marques Ventura (IF/00402/2015)
agradecimentos
This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, FCT Ref. UID/CTM/50011/2019, financed by national funds through the FCT/MCTES. The authors acknowledge the financial support from the European Union Framework Programme for Research and Innovation HORIZON 2020, under the TEAMING Grant agreement No 739572 -The Discoveries CTR. Moreover, the authors acknowledge the Portuguese Foundation for Science and Technology (FCT) for the doctoral grant of SFRH/BD/102915/2014 of Joao H. P. M. Santos. S. P. M. Ventura acknowledges FCT for the contract IF/00402/2015. A. C. R. V. Dias and P. S. G. N. Quinteiro acknowledge FCT for the contracts CEECIND/02174/2017 and CEECIND/00143/2017, respectively, and for the financial support to CESAM (UID/AMB/50017/2019), through national funds. The authors are grateful for the financial support of Sao Paulo Research Foundation - FAPESP (grant 2016/22065-5) and the National Council for Scientific and Technological Development-CNPq, the Coordination of Improvement of Higher Level Personnel-CAPES (Project 001).