resumo
This work assesses the urinary metabolite signature of prematurity in newborns by nuclear magnetic resonance (NMR) spectroscopy, while establishing the role of possible confounders and signature specificity, through comparison to other disorders. Gender and delivery mode are shown to impact importantly on newborn urine composition, their analysis pointing out at specific metabolite variations requiring consideration in unmatched subject groups. Premature newborns are, however, characterized by a stronger signature of varying metabolites, suggestive of disturbances in nucleotide metabolism, lung surfactants biosynthesis and renal function, along with enhancement of tricarboxylic acid (TCA) cycle activity, fatty acids oxidation, and oxidative stress. Comparison with other abnormal conditions (respiratory depression episode, large for gestational age, malformations, jaundice and premature rupture of membranes) reveals that such signature seems to be largely specific of preterm newborns, showing that NMR metabolomics can retrieve particular disorder effects, as well as general stress effects. These results provide valuable novel information on the metabolic impact of prematurity, contributing to the better understanding of its effects on the newborn's state of health.
palavras-chave
INTRAUTERINE GROWTH-RETARDATION; HYPOXIC-ISCHEMIC ENCEPHALOPATHY; MAGNETIC-RESONANCE-SPECTROSCOPY; NMR-BASED METABONOMICS; BIRTH-WEIGHT INFANTS; OXIDATIVE STRESS; INBORN-ERRORS; PRETERM NEWBORNS; H-1-NMR; PREGNANCY
categoria
Biochemistry & Molecular Biology
autores
Diaz, SO; Pinto, J; Barros, AS; Morais, E; Duarte, D; Negrao, F; Pita, C; Almeida, MD; Carreira, IM; Spraul, M; Gil, AM
nossos autores
Ana Maria Pissarra Coelho Gil
Professor Associado com Agregação
António de Sousa Barros
Bolseiro de pós-Doutoramento
Daniela Duarte
Estudante de doutoramento
Elisabete Cristina Cordeiro Morais
Estudante de doutoramento
Joana Isabel Monteiro Pinto
Bolseiro de pós-Doutoramento
Sílvia de Oliveira Diaz
Estudante de doutoramentoagradecimentos
This work was developed within the scope of the project CICECO-Aveiro Institute of Materials (FCT UID/CTM/50011/2013), financed by national funds through the FCT/MEC and cofinanced by FEDER under the PT2020 Partnership Agreement and QOPNA FCT-Funding: PEst-C/QUI/UI0062/2013. S.D. thanks FCT for the SFRH/BD/64159/2009 grant. JP thanks FCT for the SFRH/BD/73343/2010 and Bruker BioSpin grants. D.D. acknowledges funds from the Observatoire Hommes-Milieux International (OHM.I)