resumo
BACKGROUNDMonoclonal antibodies are becoming a leading class of biopharmaceuticals but to increase their accessibility by the general population, new production processes must be developed in particular for the downstream processing. RESULTSIn this work, an alternative and innovative affinity chromatographic method using quaternary amine matrices is proposed. Separation is driven by the dual affinity ligand LYTAG-Z, composed of a choline binding polypeptide (LYTAG) and the synthetic antibody binding Z domain. A two-elution method was developed for the purification of mAbs and the performance of different anion exchangers containing quaternary amines that act as choline analogues-CIMmultus Q, Q Sepharose and gPore Q-were tested and compared, with both CIMmultus Q and Q Sepharose allowing a recovery of more than 94% of mAbs from a CHO cell supernatant with a purity greater than 95%. An integrated platform combining an initial affinity extraction step for the clarification and capture of mAbs and a subsequent chromatographic separation using Q-matrices for the polishing of mAbs is also proposed. LYTAG-Z triggers the extraction of 94.71.7% mAbs to the PEG-rich phase, as opposed to 26.9 +/- 0.6% in the absence of the ligand, using 7% PEG 3350 and 6% dextran 500k. Further purification using Q Sepharose allowed a mAb recovery of 95.3 +/- 1.4% with a purity level of 91.4 +/- 13.0%. CONCLUSIONAn integrated platform based on two purification steps-affinity extraction and affinity chromatography-results in an overall process yield of 90%, allowing the processing of mAbs directly from a non-clarified CHO cell culture. (c) 2017 Society of Chemical Industry
palavras-chave
AQUEOUS 2-PHASE SYSTEMS; HIGH-CAPACITY BIOSEPARATIONS; ENHANCED PURIFICATION; PROCESS PERFORMANCE; FIBROUS ADSORBENTS; MAJOR AUTOLYSIN; HIGH-THROUGHPUT; BINDING; CHROMATOGRAPHY; BIOPHARMACEUTICALS
categoria
Biotechnology & Applied Microbiology; Chemistry; Engineering
autores
Campos-Pinto, I; Capela, EV; Silva-Santos, AR; Rodriguez, MA; Gavara, PR; Fernandez-Lahore, M; Aires-Barros, MR; Azevedo, AM
nossos autores
Emanuel V. Capela
Investigador Júnioragradecimentos
This work was funded by the European Union 7th framework program under grant agreement no. 312004. IBB thanks Fundacao para a Ciencia e Tecnologia (FCT) for funding through the research unit (FCT Ref. UID/BIO/04565/2013) and project PureMab (FCT Ref. PTDC/QEQ-PRS/0286/2014). E.V. Capela and A.M. Azevedo also thanks FCT for the PhD grant SFRH/BD/126202/2016 and the research contract under the program 'Investigador FCT 2014' (IF/00048/2014/CP1214/CT0010), respectively.