resumo
Mussels are marine organisms that have been mimicked due to their exceptional adhesive properties to all kind of surfaces, including rocks, under wet conditions. The proteins present on the mussel's foot contain 3,4-dihydroxy-L-alanine (DOPA), an amino acid from the catechol family that has been reported by their adhesive character. Therefore, we synthesized a mussel-inspired conjugated polymer, modifying the backbone of hyaluronic acid with dopamine by carbodiimide chemistry. Ultraviolet-visible (UV-Vis) spectroscopy and nuclear magnetic resonance (NMR) techniques confirmed the success of this modification. Different techniques have been reported to produce two-dimensional (2D) or three-dimensional (3D) systems capable to support cells and tissue regeneration; among others, multilayer systems allow the construction of hierarchical structures from nano-to macroscales. In this study, the layer-by-layer (LbL) technique was used to produce freestanding multilayer membranes made uniquely of chitosan and dopamine-modified hyaluronic acid (HA-DN). The electrostatic interactions were found to be the main forces involved in the film construction. The surface morphology, chemistry, and mechanical properties of the freestanding membranes were characterized, confirming the enhancement of the adhesive properties in the presence of HA-DN. The MC3T3-E1 cell line was cultured on the surface of the membranes, demonstrating the potential of these freestanding multilayer systems to be used for bone tissue engineering.
palavras-chave
MUSSEL ADHESION; BIOMEDICAL APPLICATIONS; OSTEOGENIC DIFFERENTIATION; SURFACE MODIFICATION; INSPIRED POLYMERS; HYALURONIC-ACID; MC3T3-E1 CELLS; CHITOSAN; FILMS; HYDROGELS
categoria
Engineering
autores
Sousa, MP; Mano, JF
nossos autores
João Mano
Vice-Diretor
Maria Sousa
Estudante de doutoramentoagradecimentos
M.P.S. acknowledges the Portuguese Foundation for Science and Technology (FCT) for financial support through Grant No. SFRH/BD/97606/2013. This work was supported by the European Research Council grant agreement ERC-2014-ADG-669858 for the ATLAS project. The authors acknowledge Carmen Freire (CICECO, University of Aveiro, Aveiro, Portugal) for providing the Instron equipment to carry out the lap shear adhesion tests.