authors |
Belchior, DCV; Almeida, MR; Sintra, TE; Ventura, SPM; Duarte, IF; Freire, MG |
nationality |
International |
journal |
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH |
keywords |
2-PHASE SYSTEMS; SALTING-OUT; 1-BUTYL-3-METHYLIMIDAZOLIUM BROMIDE; PROTEIN SEPARATION; SELF-AGGREGATION; PHASE-SEPARATION; CATION SYMMETRY; WATER; CHOLINE; IMPACT |
abstract |
Aqueous biphasic systems constituted by ionic liquids (IL-based ABSs) are a target of investigation in the separation of high-value biomolecules. However, identification of the molecular-level mechanisms ruling the two-phase formation and extraction performance of these systems is crucial to the successful design of effective separation processes. In this work, IL-based ABSs formed by K2HPO4 and cholinium carboxylate ILs ([Ch] [CnCO2] with n = 1-7, comprising anions with odd and even alkyl chain lengths) were investigated. The corresponding ternary phase diagrams, including binodal curves, tie-lines, tie-line lengths, and critical points, as well as the Setschenow salting-out coefficients (k(s)), which quantitatively describe the two-phase formation ability, were determined at 298 K. The extraction capability of these systems was then evaluated for four amino acids (L-tryptophan, L-phenylalanine, L-tyrosine, and L-3,4-dihydroxyphenylalanine/L-dopa). It was found that ILs composed of anions with even alkyl chains display slightly higher k(s) values, meaning that these ILs are more easily salted out or more easily phase-separated to form ABSs, whereas ABSs formed by ILs with anions comprising odd alkyl chains lead to slightly higher partition coefficients of amino acids. Beyond the neat IL odd-even effect resulting from their nanostructuration, being this a well-known phenomenon occurring in a series of their thermophysical properties, the existence of an odd-even effect displayed by the IL anion aliphatic moiety in aqueous solution is shown here, visible in both the two-phase formation ability and extraction performance of ABSs. These findings contribute to elucidate of the molecular-level mechanisms governing ABS formation and partitioning of biomolecules, ultimately contributing to the design of proficient separation platforms. |
publisher |
AMER CHEMICAL SOC |
issn |
0888-5885 |
year published |
2019 |
volume |
58 |
issue |
19 |
beginning page |
8323 |
ending page |
8331 |
digital object identifier (doi) |
10.1021/acs.iecr.9b00663 |
web of science category |
Engineering, Chemical |
subject category |
Engineering |
unique article identifier |
WOS:000468368100068
|
ciceco authors
impact metrics
journal analysis (jcr 2019):
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journal impact factor |
3.573 |
5 year journal impact factor |
3.684 |
category normalized journal impact factor percentile |
69.58 |
dimensions (citation analysis):
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altmetrics (social interaction):
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