resumo
The build-up and degradation of cytocompatible nanofilms in a controlled fashion have great potential in biomedical and nanomedicinal fields, including single-cell nanoencapsulation (SCNE). Herein, we report the fabrication of biodegradable films of cationic starch (c-ST) and anionic alginate (ALG) by electrostatically driven layer-by-layer (LbL) assembly technology and its application to the SCNE. The [c-ST/ALG] multilayer nanofilms, assembled either on individual Saccharomyces cerevisiae or on the 2D flat gold surface, degrade on demand, in a cytocompatible fashion, via treatment with alpha-amylase. Their degradation profiles are investigated, while systematically changing the alpha-amylase concentration, by several surface characterization techniques, including quartz crystal microbalance with dissipation monitoring (QCM-D) and ellipsometry. DNA incorporation in the LbL nanofilms and its controlled release, upon exposure of the nanofilms to an aqueous alpha-amylase solution, are demonstrated. The highly cytocompatible nature of the film-forming and -degrading conditions is assessed in the c-ST/ALG-shell formation and degradation of S. cerevisiae. We envisage that the cytocompatible, enzymatic degradation of c-ST-based nanofilms paves the way for developing advanced biomedical devices with programmed dissolution in vivo.
palavras-chave
INDIVIDUAL MAMMALIAN-CELLS; BIOMIMETIC COATINGS; MULTILAYER FILMS; LINEAR GROWTH; CHITOSAN; SHELLS; MICROORGANISMS; ENCAPSULATION; FABRICATION; DELIVERY
categoria
Chemistry; Materials Science; Physics; Polymer Science
autores
Moon, HC; Han, S; Borges, J; Pesqueira, T; Choi, H; Han, SY; Cho, H; Park, JH; Mano, JF; Choi, IS
nossos autores
João Borges
Investigador Auxiliar
João Mano
Vice-DiretorProjectos
agradecimentos
This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2018R1C1B5045778), Programa Operacional Regional do Centro -Centro 2020, in the component FEDER, and by national funds (OE) through FCT/MCTES, in the scope of the project ``SUPRASORT'' (PTDC/QUI-OUT/30658/2017, CENTRO-01-0145-FEDER-030658), as well as by the European Research Council grant agreement ERC-2014-ADG-669858 (ATLAS). J. Borges gratefully acknowledges FCT for the individual contract (CEECIND/03202/2017). This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020 & UIDP/50011/2020, financed by national funds through the Foundation for Science and Technology/MCTES.