Enzymatically degradable, starch-based layer-by-layer films: application to cytocompatible single-cell nanoencapsulation

authors	Moon, HC; Han, S; Borges, J; Pesqueira, T; Choi, H; Han, SY; Cho, H; Park, JH; Mano, JF; Choi, IS
nationality	International
journal	SOFT MATTER
keywords	INDIVIDUAL MAMMALIAN-CELLS; BIOMIMETIC COATINGS; MULTILAYER FILMS; LINEAR GROWTH; CHITOSAN; SHELLS; MICROORGANISMS; ENCAPSULATION; FABRICATION; DELIVERY
abstract	The build-up and degradation of cytocompatible nanofilms in a controlled fashion have great potential in biomedical and nanomedicinal fields, including single-cell nanoencapsulation (SCNE). Herein, we report the fabrication of biodegradable films of cationic starch (c-ST) and anionic alginate (ALG) by electrostatically driven layer-by-layer (LbL) assembly technology and its application to the SCNE. The [c-ST/ALG] multilayer nanofilms, assembled either on individual Saccharomyces cerevisiae or on the 2D flat gold surface, degrade on demand, in a cytocompatible fashion, via treatment with alpha-amylase. Their degradation profiles are investigated, while systematically changing the alpha-amylase concentration, by several surface characterization techniques, including quartz crystal microbalance with dissipation monitoring (QCM-D) and ellipsometry. DNA incorporation in the LbL nanofilms and its controlled release, upon exposure of the nanofilms to an aqueous alpha-amylase solution, are demonstrated. The highly cytocompatible nature of the film-forming and -degrading conditions is assessed in the c-ST/ALG-shell formation and degradation of S. cerevisiae. We envisage that the cytocompatible, enzymatic degradation of c-ST-based nanofilms paves the way for developing advanced biomedical devices with programmed dissolution in vivo.
publisher	ROYAL SOC CHEMISTRY
issn	1744-683X
isbn	1744-6848
year published	2020
volume	16
issue	26
beginning page	6063
ending page	6071
digital object identifier (doi)	10.1039/d0sm00876a
web of science category	Chemistry, Physical; Materials Science, Multidisciplinary; Physics, Multidisciplinary; Polymer Science
subject category	Chemistry; Materials Science; Physics; Polymer Science
unique article identifier	WOS:000552440400021