authors |
Moon, HC; Han, S; Borges, J; Pesqueira, T; Choi, H; Han, SY; Cho, H; Park, JH; Mano, JF; Choi, IS |
nationality |
International |
journal |
SOFT MATTER |
keywords |
INDIVIDUAL MAMMALIAN-CELLS; BIOMIMETIC COATINGS; MULTILAYER FILMS; LINEAR GROWTH; CHITOSAN; SHELLS; MICROORGANISMS; ENCAPSULATION; FABRICATION; DELIVERY |
abstract |
The build-up and degradation of cytocompatible nanofilms in a controlled fashion have great potential in biomedical and nanomedicinal fields, including single-cell nanoencapsulation (SCNE). Herein, we report the fabrication of biodegradable films of cationic starch (c-ST) and anionic alginate (ALG) by electrostatically driven layer-by-layer (LbL) assembly technology and its application to the SCNE. The [c-ST/ALG] multilayer nanofilms, assembled either on individual Saccharomyces cerevisiae or on the 2D flat gold surface, degrade on demand, in a cytocompatible fashion, via treatment with alpha-amylase. Their degradation profiles are investigated, while systematically changing the alpha-amylase concentration, by several surface characterization techniques, including quartz crystal microbalance with dissipation monitoring (QCM-D) and ellipsometry. DNA incorporation in the LbL nanofilms and its controlled release, upon exposure of the nanofilms to an aqueous alpha-amylase solution, are demonstrated. The highly cytocompatible nature of the film-forming and -degrading conditions is assessed in the c-ST/ALG-shell formation and degradation of S. cerevisiae. We envisage that the cytocompatible, enzymatic degradation of c-ST-based nanofilms paves the way for developing advanced biomedical devices with programmed dissolution in vivo. |
publisher |
ROYAL SOC CHEMISTRY |
issn |
1744-683X |
isbn |
1744-6848 |
year published |
2020 |
volume |
16 |
issue |
26 |
beginning page |
6063 |
ending page |
6071 |
digital object identifier (doi) |
10.1039/d0sm00876a |
web of science category |
Chemistry, Physical; Materials Science, Multidisciplinary; Physics, Multidisciplinary; Polymer Science |
subject category |
Chemistry; Materials Science; Physics; Polymer Science |
unique article identifier |
WOS:000552440400021
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ciceco authors
impact metrics
journal analysis (jcr 2019):
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journal impact factor |
3.14 |
5 year journal impact factor |
3.466 |
category normalized journal impact factor percentile |
66.619 |
dimensions (citation analysis):
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altmetrics (social interaction):
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