authors |
Cicuendez, M; Coimbra, A; Santos, J; Oliveira, H; Ayan-Varela, M; Paredes, UI; Villar-Rodil, S; Vila, M; Silva, VS |
nationality |
International |
journal |
ACS APPLIED BIO MATERIALS |
author keywords |
pristine graphene; flavin mononucleotide; in vitro cell response; ROS; osteoblasts |
keywords |
NANOMATERIALS; APOPTOSIS; OXIDE; THERAPEUTICS; GENERATION; PRISTINE; HYBRIDS; STRESS; FLAKES |
abstract |
An appealing strategy that overcomes the hydrophobicity of pristine graphene and favors its interaction with biological media is colloidal stabilization in aqueous medium with the support of a biomolecule, such as flavin mononucleotide (FMN), as exfoliating/dispersing agent. However, to establish FMN-stabilized graphene (PG-FMN) as suitable for use in biomedicine, its biocompatibility must be proved by a complete assessment of cytotoxicity at the cellular level. Furthermore, if PG-FMN is to be proposed as a theranostic agent, such a study should include both healthy and tumoral cells and its outcome should reveal the nanomaterial as selectively toxic to the latter. Here, we provide an in-depth comparative in vitro analysis of the response of Saos-2 human sarcoma osteoblasts (model tumor cells) and MC3T3-E1 murine preosteoblasts (undifferentiated healthy cells) upon incubation with different concentrations (10-50 mu g mL(-1)) of PG-FMN dispersions constituted by flakes with different average lateral size (90 and 270 nm). Specifically, the impact of PG-FMN on the viability and cell proliferation, reactive oxygen species (ROS) production, and the cellular incorporation process, cell-cycle progression, and apoptosis has been evaluated. PG-FMN was found to be toxic to both types of cells by increasing ROS production and triggering cell-cycle arrest. The present results constitute a cautionary tale on the need to establish the effect of a nanomaterial not only on tumor cells but also on healthy ones before proposing it as anticancer agent. |
publisher |
AMER CHEMICAL SOC |
issn |
2576-6422 |
year published |
2021 |
volume |
4 |
issue |
5 |
beginning page |
4384 |
ending page |
4393 |
digital object identifier (doi) |
10.1021/acsabm.1c00144 |
web of science category |
10 |
subject category |
Nanoscience & Nanotechnology; Materials Science, Biomaterials |
unique article identifier |
WOS:000653545300054
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ciceco authors
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