resumo
Ionic liquids (ILs) are salts with low melting points that can be used as solvents for mild extraction and selective fractionation of biomolecules (e.g., proteins, carbohydrates, lipids, and pigments), enabling the valorisation of microalgal biomass in a multiproduct biorefinery concept, while maintaining the biomolecules' structural integrity and activity. Aqueous biphasic systems and emulsions stabilised by core-shell particles have been used to fractionate disrupted microalgal biomass into hydrophobic (lipids and pigments) and hydrophilic (proteins and carbohydrates) components. From nondisrupted biomass, the hydrophobic components can be directly extracted using ILs from intact cells, while the most fragile hydrophilic components can be obtained upon further mechanical cell disruption. These multiproduct biorefinery concepts will be discussed in an outlook on future separations using IL-based systems.
palavras-chave
DEEP EUTECTIC SOLVENTS; SELECTIVE ISOLATION; LIPID EXTRACTION; PROCESS DESIGN; PROTEINS; FRACTIONATION; SEPARATION; STABILITY; TOXICITY; PURIFICATION
categoria
Biotechnology & Applied Microbiology
autores
Eppink, MHM; Ventura, SPM; Coutinho, JAP; Wijffels, RH
nossos autores
Sónia Patrícia Marques Ventura
Professor Auxiliaragradecimentos
This work is performed within the TKI AlgaePARC Biorefinery program with financial support from The Netherlands' Ministry of Economic Affairs in the framework of the TKI BioBased Economy under contract nr. TKIBE01009 and within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020 and UIDP/50011/2020, financed by national funds through the Portuguese Foundation for Science and Technology/MCTES.