A comprehensive study of the structure and piezoelectric response of biodegradable polyhydroxybutyrate-based films for tissue engineering applications

resumo

The results of comprehensive research on the thermal behavior and molecular and crystalline structures of poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-HV) films of different thicknesses, their molecular weights (M-W) and 3-hydroxyvalerate (3-HV) contents are reported. Increasing film thickness from 30 to 100 mu m resulted in an isotropic crystal orientation, reducing the crystallite size of the orthorhombic alpha-phase in the b direction from 22 to 17 nm and increasing the degree of crystallinity of the PHB films without affecting their thermal behavior. Furthermore, despite resulting in the same degree of crystallinity and roughness, an similar to 8-fold decrease in PHB M-W from 803 kDa to 102 kDa resulted in a decreased number of piezoactive domains. The addition of 5.9% 3-HV resulted in anisotropy in the PHB crystalline structure and increased D-(020) from 19 nm to 24 nm. Additionally, a further increase in the 3-HV content to 17.5% in the PHB-HV films led to a decrease in the melting temperature and a decrease in the degree of crystallinity from 57% to 23%, which resulted in the absence of local piezoresponse. Notably, the decrease in the M-W of PHB-HV (similar to 17%) from 1177 kDa to 756 kDa resulted in an increase in the degree of crystallinity from 23% to 32%. Moreover, the PHB-HV films became smoother with increasing 3-HV content.

palavras-chave

O HYDROGEN BONDINGS; INFRARED-SPECTROSCOPY; THERMAL-BEHAVIOR; ENZYMATIC DEGRADATION; CONTROLLED-RELEASE; MELTING BEHAVIOR; POLY(3-HYDROXYBUTYRATE); ACID); PHB; POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)

categoria

Polymer Science

autores

Chernozem, RV; Pariy, IO; Pryadko, A; Bonartsev, AP; Voinova, VV; Zhuikov, VA; Makhina, TK; Bonartseva, GA; Shaitan, KV; Shvartsman, VV; Lupascu, DC; Romanyuk, KN; Kholkin, AL; Surmenev, RA; Surmeneva, MA

Grupos

G2 - Materiais Fotónicos, Eletrónicos e Magnéticos

Projectos

CICECO - Aveiro Institute of Materials (UIDB/50011/2020)

CICECO - Aveiro Institute of Materials (UIDP/50011/2020)

Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)

Collaboratory for Emerging Technologies, CoLab (EMERGING TECHNOLOGIES)

agradecimentos

This research was performed at Tomsk Polytechnic University within the framework of the Tomsk Polytechnic University Development Program (infrastructure provided for research activities) and was financially supported by the Russian Science Foundation (project number 20-63-47096, sample fabrication and characterization of their properties) and Ministry of Science and Higher Education (#075-15-2021-588 from 1.06.2021, piezoresponse study). RC, VS and RS acknowledge support from the German-Russian Interdisciplinary Science Center (G-risc) funded by the German Academic Exchange Service (DAAD). This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020, UIDP/50011/2020 & LA/P/0006/2020, financed by national funds through the FCT/MEC (PIDDAC). We also acknowledge Alexey Rusakov and Alexey Useinov from the Federal State Budgetary Institution Technological Institute for Superhard and Novel Carbon Materials for their help in performing previous measurements of the Young's modulus of the PHB and PHB-HV films by nanoindentation.