resumo
The increasing use of molybdenum disulfide (MoS2) nanoparticles (NPs) raises concerns regarding their accumulation in soil ecosystems, with limited studies on their impact on soil organisms. Study aim: To unravel the effects of MoS2 nanosheets (two-dimensional (2D) MoS2 NPs) and bulk MoS2 (156, 313, 625, 1250, 2500 mg/kg) on Enchytraeus crypticus and Folsomia candida. The organisms' survival and avoidance behavior remained unaffected by both forms, while reproduction and DNA integrity were impacted. For E. crypticus, the individual endpoint reproduction was more sensitive, increasing at lower concentrations of bulk MoS2 and decreasing at higher ones and at 625 mg/kg of 2D MoS2 NPs. For F. candida, the molecular endpoint DNA integrity was more impacted: 2500 mg/kg of bulk MoS2 induced DNA damage after 2 days, with all concentrations inducing damage by day 7. 2D MoS2 NPs induced DNA damage at 156 and 2500 mg/kg after 2 days, and at 1250 and 2500 mg/kg after 7 days. Despite affecting the same endpoints, bulk MoS2 induced more effects than 2D MoS2 NPs. Indeed, 2D MoS2 NPs only inhibited E. crypticus reproduction at 625 mg/kg and induced fewer (F. candida) or no effects (E. crypticus) on DNA integrity. This study highlights the different responses of terrestrial organisms to 2D MoS2 NPs versus bulk MoS2, reinforcing the importance of risk assessment when considering both forms.
palavras-chave
ZNO NANOPARTICLES; FOLSOMIA-CANDIDA; TOXICITY; NANO
categoria
Chemistry; Science & Technology - Other Topics; Materials Science; Physics
autores
Santos, J; Barreto, A; Fernandes, C; Silva, ARR; Cardoso, DN; Pinto, E; Daniel-da-Silva, AL; Maria, VL
nossos autores
Ana Luísa Daniel da Silva
Investigador Principal
Joana Patrícia da Silva Santos
Estudante de doutoramentoProjectos
CICECO - Aveiro Institute of Materials (UIDB/50011/2020)
CICECO - Aveiro Institute of Materials (UIDP/50011/2020)
Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)
agradecimentos
This research was funded by FCT/MCTES through national funds (PIDDAC) and co-funded by FEDER within the PT2020 Partnership Agreement and Compete 2020 by CESAM (UIDP/50017/2020 + UIDB/50017/2020 + LA/P/0094/2020). This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020, UIDP/50011/2020 and LA/P/0006/2020, financed by national funds through the FCT/MEC (PIDDAC). J. Santos, A. Barretoand D. Cardoso were funded by FCT via 2021.04771.BD, CEECIND/01275/2018 and CEECIND/01190/2018, respectively. V. L. Maria was funded by national funds (OE) through FCT in the scope of the framework contract foreseen in numbers 4, 5 and 6 of article 23 of the Decree Law 57/2016 fromAugust 29, changed by Law 57/2017 from July 19.