Metal-Organic Frameworks as Sensors for Human Amyloid Diseases

resumo

Metal-organic frameworks (MOFs) are versatile compounds with emergent applications in the fabrication of biosensors for amyloid diseases. They hold great potential in biospecimen protection and unprecedented probing capabilities for optical and redox receptors. In this Review, we summarize the main methodologies employed in the fabrication of MOF-based sensors for amyloid diseases and collect all available data in the literature related to their performance (detection range, limit of detection, recovery, time of analysis, among other parameters). Nowadays, MOF sensors have evolved to a point where they can, in some cases, outperform technologies employed in the detection of several amyloid biomarkers (amyloid # peptide, a-synuclein, insulin, procalcitonin, and prolactin) present in biological fluids, such as cerebrospinal fluid and blood. A special emphasis has been given by researchers on Alzheimer's disease monitoring to the detriment of other amyloidosis that are underexploited despite their societal relevance (e.g., Parkinson's disease). There are still important obstacles to overcome in order to selectively detect the various peptide isoforms and soluble amyloid species associated with Alzheimer's disease. Furthermore, MOF contrast agents for imaging peptide soluble oligomers in living humans are also scarce (if not nonexistent), and action in this direction is unquestionably required to clarify the contentious link between the amyloidogenic species and the disease, guiding research toward the most promising therapeutic strategies.

palavras-chave

ALZHEIMERS-DISEASE; BETA-PEPTIDE; ZINC-BINDING; MOF; IMMUNOASSAY; HYPOTHESIS; APTASENSOR; STABILITY; DIAGNOSIS; OLIGOMER

categoria

Chemistry; Science & Technology - Other Topics

autores

Leite, JP; Figueira, F; Mendes, RF; Paz, FAA; Gales, L

Grupos

G1 - Materiais Porosos e Nanossistemas

Projectos

CICECO - Aveiro Institute of Materials (UIDB/50011/2020)

CICECO - Aveiro Institute of Materials (UIDP/50011/2020)

Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)

agradecimentos

Agencies and Projects: This work was funded by (i) FEDER- Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020 Operational Programme for Competitiveness and Internationalisation (POCI), Portugal 2020, andby Portuguese funds through FCT/MCTES in the framework of project POCI-01-0145-FEDER-007274 (Institute for Research and Innovation in Health Sciences) , and by (ii) FEDER through Norte Portugal Regional Operational Programme (NORTE 2020) , under the PORTUGAL 2020 Partnership Agreement in the framework of Projects Norte-01-0145-FEDER-000008. This work was developed within the scope of the project CICECO-Aveiro Institute of Materials (UIDB/50011/2020, UIDP/50011/2020, and LA/P/0006/2020) , financed by national funds through the Portuguese Foundation for Science and Technology/MCTES, and PTDC/QUI-QFI/29914/2017, financed by national funds through the FCT/MCTES. The work was also supported by UID/MULTI/04378/2019 and UID/QUI/00081 with fund-ing from FCT/MCTES through national funds. Individual Grants and Scholarships: FCT is gratefully acknowledged for the PhD grant SFRH/BD/129921/2017 (to J.P.L.) and the Junior Research Position CEECIND/00553/2017 (to R.F.M.) . The research contract of F.F. (REF-168-89-ARH/2018) is funded by national funds (OE) , through FCT, in the scope of the framework contract foreseen in Nos. 4, 5, and 6 of article 23 of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19.