abstract
Aiming at developing not only cheaper but also biocompatible and sustainable extraction and purification processes for antibiotics, in this work it was evaluated the ability of aqueous two-phase systems (ATPS) composed of polyethylene glycol (PEG) and cholinium-based salts to extract tetracycline from the fermented broth of Streptomyces aureofaciens. Conventional polymer/salt and salt/salt ATPS were also studied for comparison purposes. The novel systems here proposed are able to extract tetracycline directly from the fermentation broth with extraction efficiencies higher than 80%. A tailored extraction ability of these systems can also be achieved, with preferential extractions either for the polymer- or salt-rich phases, and which further depend on the cholinium-based salt employed. The gathered results support the applicability of biocompatible ATPS in the extraction of antibiotics from complex matrices and can be envisaged as valuable platforms to be applied at the industrial level by pharmaceutical companies. (c) 2013 Elsevier Ltd. All rights reserved.
keywords
BIPHASIC SYSTEMS; IONIC LIQUIDS; HYDROCHLORIDE FORMS; HOFMEISTER SERIES; ANTIBIOTICS; CHROMATOGRAPHY; CIPROFLOXACIN; SOLUBILITY
subject category
Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering
authors
Pereira, JFB; Vicente, F; Santos-Ebinuma, VC; Araujo, JM; Pessoa, A; Freire, MG; Coutinho, JAP
our authors
Jorge Fernando Brandão Pereira
PhD Student
Mara Guadalupe Freire Martins
Coordinating ResearcherGroups
G4 - Renewable Materials and Circular Economy
G5 - Biomimetic, Biological and Living Materials
Projects
acknowledgements
This work was financed by national funding from FCT-Fundacao para a Ciencia e a Tecnologia, through the projects PTDC/QUI-QUI/121520/2010 and Pest-C/CTM/LA0011/2011. The authors also acknowledge FCT for the doctoral and post-doctoral grants SFRH/BD/60228/2009 and SFRH/BPD/41781/2007 of J.F.B. Pereira and M.G. Freire, respectively. V.C.S. Ebinuma and A. Pessoa knowledge the financial support of FAPESP (Sao Paulo Research Foundation, Brazil), CNPq (National Council for Scientific and Technological Development-Brazil) and CAPES (Coordination of Superior Level Staff Improvement, Brazil).