abstract
Rubberwood (Hevea brasiliensis), a potential raw material for bioethanol production due to its high cellulose content, was used as a novel feedstock for enzymatic hydrolysis and bioethanol production using biological pretreatment. To improve ethanol production, rubberwood was pretreated with white rot fungus Ceriporiopsis subvermispora to increase fermentation efficiency. The effects of particle size of rubberwood (1 mm, 0.5 mm, and 0.25 mm) and pretreatment time on the biological pretreatment were first determined by chemical analysis and X-ray diffraction and their best condition obtained with 1mm particle size and 90 days pretreatment. Further morphological study on rubberwood with 1mm particle size pretreated by fungus was performed by FTIR spectra analysis and SEM observation and the result indicated the ability of this fungus for pretreatment. A study on enzymatic hydrolysis resulted in an increased sugar yield of 27.67% as compared with untreated rubberwood (2.88%). The maximum ethanol concentration and yield were 17.9 g/L and 53% yield, respectively, after 120 hours. The results obtained demonstrate that rubberwood pretreated by C. subvermispora can be used as an alternative material for the enzymatic hydrolysis and bioethanol production.
keywords
WHITE-ROT FUNGI; ETHANOL-PRODUCTION; MICROBIAL PRETREATMENT; CORN STOVER; BEECH WOOD; SIMULTANEOUS SACCHARIFICATION; PHANEROCHAETE-CHRYSOSPORIUM; COTTON STALKS; FERMENTATION; FEATURES
subject category
Biotechnology & Applied Microbiology; Research & Experimental Medicine
authors
Nazarpour, F; Abdullah, DK; Abdullah, N; Motedayen, N; Zamiri, R
our authors
Reza Zamiri
Post-doc FellowshipGroups
acknowledgements
The authors wish to thank to the Laboratory of Industrial Biotechnology, Institute of Bioscience, Universiti Putra Malaysia for providing materials and facilities. Also we sincerely thank the staff of the Microscopy Unit at the Institute of Bioscience, Universiti Putra Malaysia for helpful assistance in the scanning electron microscopy assays. The author Reza Zamiri would like to express his personal thanks to FCT (Fundacao para a Ciencia e a Tecnologia) for post-doctoral research grant with reference number (SFRH/BPD/76185/2011).