Superactivity of alpha-chymotrypsin with biological buffers, TRIS, TES, TAPS, and TAPSO in aqueous solutions

abstract

Biological buffers are always considered as non-toxic, biocompatible and green compounds. Therefore, we analyzed the catalytic activity of a commercial enzyme alpha-chymotrypsin (alpha-CT) in aqueous solutions of some common biological buffers (TRIS, TES, TAPS, and TAPSO) at pH 8 and T = 25 degrees C. It is found that the increase of the buffer concentration enhanced the catalytic activity of enzyme alpha-CT, and the tendency follows the order of TRIS > TES > TAPS > TAPSO. Especially in the presence of TRIS, the catalytic activity enhanced 5.5 fold.

keywords

BOVINE SERUM-ALBUMIN; IONIC LIQUIDS; ORGANIC MEDIA; WATER; STABILITY; ENZYME; FLUORESCENCE; STABILIZATION; COMPLEXES; PROTEINS

subject category

Chemistry

authors

Gupta, BS; Taha, M; Lee, MJ

our authors

Groups

acknowledgements

The authors gratefully acknowledge the financial support from the National Science Council, Taiwan, through Grant no. NSC102-2811-E-011-014 and NSC 102-2221-E011-137-MY3.

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