abstract
Carotenoids are lipophilic compounds and their production is one of the most challenging, yet rewarding, activities in algal biotechnology. Some carotenoids (fucoxanthin included) have antioxidant activity and many studies have confirmed their health benefits. Fucoxanthin is considered as one of the most important intracellular active metabolites present in brown macroalgae. In this work, aqueous solutions of different surface-active ionic liquids and anionic surfactants were evaluated for the carotenoids extraction. Aqueous solutions of sodium dodecyl sulfate were selected as the media with the best extractive performance. For the best solvent, the solid-liquid ratio, concentration, and time of extraction were the conditions optimized and the maximum yield of extraction of carotenoids attained found to be between 2.57 +/- 0.26 mg(carotenoids)/g(dried) mass and 3.31 +/- 0.02 g M mg(carotenoids)/g(dried) mass, depending of the algae batch used. This said, this work proposed an efficient water-based process of carotenoids extraction, for both dried (more 37.4% of carotenoids extracted) and wet (more 30.2% of carotenoids extracted) biomass matrices. (C) 2017 Elsevier B.V. All rights reserved.
keywords
SODIUM DODECYL-SULFATE; FUCOXANTHIN; EXTRACTION; BROMIDE; ALGAE
subject category
Engineering
authors
Vieira, FA; Guilherme, RJR; Neves, MC; Rego, A; Abreu, MH; Coutinho, JAP; Ventura, SPM
our authors
Flávia Aparecida Vieira
Post-Doc Fellowship
Márcia Carvalho Neves
Assistant Researcher
Sónia Patrícia Marques Ventura
Assistant ProfessorGroups
G4 - Renewable Materials and Circular Economy
G5 - Biomimetic, Biological and Living Materials
Projects
CICECO - Aveiro Institute of Materials (UID/CTM/50011/2013)
EXIL - Exchange on Ionic Liquids (EXIL)
Projeto de Investigação Exploratória: Sónia Patricia Marques Ventura (IF/00402/2015)
acknowledgements
The authors are grateful for the financial support of international funding from the program Ciencias sem Fronteiras (Brazil) through the post-doctoral grant of Flavia Aparecida Vieira, process number 249485/2013-3. This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, POCI-01-0145-FEDER-007679 (FCT Ref. UID/CTM/50011/2013), financed by national funds through the FCT/MEC and when appropriate co-financed by FEDER under the PT2020 Partnership Agreement. The authors would like to acknowledge COST for funding STSM within CM1206 action (Ref. COST-STSM-ECOST-STSM-CM1206-200114-0 40305). The authors thank FCT for the post-doctoral grant SFRH/BPD/110423/2015 of Marcia C. Neves. Sonia P.M. Ventura acknowledges FCT for the IF contract IF/00402/2015 under the Investigador FCT 2015 program. ALGApIus activities were supported by the project SEACOLORS, LIFE13 ENV/ES/000445.