Fluorescent Light-up Probe for the Detection of Protein Aggregates


A fluorescent dye was decorated with water-soluble pyridinium groups in order to be applied in the detection of cyclodextrins or DNA. The dye displays an enhancement of its emission intensity when the internal rotations are restricted due to the formation of an inclusion complex with cyclodextrins or upon interaction with DNA. In vivo, the fluorescent probe can stain protein aggregates with a selectivity comparable to the widely used Proteostat (R).



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da Silva, RN; Costa, CC; Santos, MJG; Alves, MQ; Braga, SS; Vieira, SI; Rocha, J; Silva, AMS; Guieu, S

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Thanks are due to University of Aveiro, FCT/MEC for the financial support to the QOPNA research Unit (FCT UID/QUI/00062/2013) and CICECO-Aveiro Institute of Materials (POCI-01-0145-FEDER-007679; FCT-UID/CTM/50011/2013), through national funds and where applicable co-financed by the FEDER, within the PT2020 Partnership Agreement, and also to the Portuguese NMR Network. This work was also supported by the Integrated Programme of SR&TD pAGEProtein aggregation Across the Lifespan (reference CENTRO-01-0145-FEDER-000003), R. Nunes da Silva Post-Doctoral grant (BPD/UI98/6327/2018), co-funded by Centro 2020 program, Portugal 2020, European Union, through the European Regional Development Fund. Ashland Specialty ingredients (Dusseldorf, Germany) is gracefully acknowledged for providing gamma-cyclodextrin. Image acquisition was performed in the LiM facility of iBiMED, a node of PPBI (Portuguese Platform of BioImaging): POCI-01-0145-FEDER-022122.

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