abstract
Magnetoliposomes containing calcium ferrite (CaFe2O4) nanoparticles were developed and characterized for the first time. CaFe2O4 nanoparticles were covered by a lipid bilayer or entrapped in liposomes forming, respectively, solid or aqueous magnetoliposomes as nanocarriers for new antitumor drugs. The magnetic nanoparticles were characterized by UV/Visible absorption, XRD, HR-TEM, and SQUID, exhibiting sizes of 5.2 +/- 1.2 nm (from TEM) and a superparamagnetic behavior. The magnetoliposomes were characterized by DLS and TEM. The incorporation of two new potential antitumor drugs (thienopyridine derivatives) specifically active against breast cancer in these nanosystems was investigated by fluorescence emission and anisotropy. Aqueous magnetoliposomes, with hydrodynamic diameters around 130 nm, and solid magnetoliposomes with sizes of ca. 170 nm, interact with biomembranes by fusion and are able to transport the antitumor drugs with generally high encapsulation efficiencies (>= 70%). These fully biocompatible drug-loaded magnetoliposomes can be promising as therapeutic agents in future applications of combined breast cancer therapy.
keywords
NILE RED; MAGNETIC NANOPARTICLES; QUANTUM YIELDS; IN-VITRO; LIPOSOMES; NANOCARRIERS; FLUORESCENCE; ANTITUMOR; CYTOTOXICITY; VESICLES
subject category
Pharmacology & Pharmacy
authors
Pereira, DSM; Cardoso, BD; Rodrigues, ARO; Amorim, CO; Amaral, VS; Almeida, BG; Queiroz, MJRP; Martinho, O; Baltazar, F; Calhelha, RC; Ferreira, ICFR; Coutinho, PJG; Castanheira, EMS
our authors
Carlos Amorim
Assistant Researcher
Vitor Brás Sequeira Amaral
Full professorGroups
G2 - Photonic, Electronic and Magnetic Materials
G6 - Virtual Materials and Artificial Intelligence
acknowledgements
This research was funded by the Portuguese Foundation for Science and Technology (FCT) in the framework of the Strategic Funding of CF-UM-UP (UID/FIS/04650/2013; UID/FIS/04650/2019), CQUM (UID/QUI/00686/2016; UID/QUI/00686/2019) and LA-26 (PEst-C/SAU/LA0026/2013), and through the research project PTDC/QUI-QFI/28020/2017 (POCI-01-0145-FEDER-028020), financed by FCT, European Fund of Regional Development (FEDER), COMPETE2020 and Portugal2020. The magnetic measurements were supported by projects UTAP-EXPL/NTec/0046/2017, NORTE-01-0145-FEDER-028538 e PTDC/FIS-MAC/29454/2017. The APC was also funded by FCT. B.D.C. acknowledges FCT for a PhD grant (SFRH/BD/141936/2018).