abstract
Measurement of thermogenesis in individual cells is a remarkable challenge due to the complexity of the biochemical environment (such as pH and ionic strength) and to the rapid and yet not well-understood heat transfer mechanisms throughout the cell. Here, we present a unique system for intracellular temperature mapping in a fluorescence microscope (uncertainty of 0.2 K) using rationally designed luminescent Ln(3+)-bearing polymeric micellar probes (Ln = Sm, Eu) incubated in breast cancer MDA-MB468 cells. Two-dimensional (2D) thermal images recorded increasing the temperature of the cells culture medium between 296 and 304 K shows inhomogeneous intracellular temperature progressions up to similar to 20 degrees and subcellular gradients of similar to 5 degrees between the nucleolus and the rest of the cell, illustrating the thermogenic activity of the different organelles and highlighting the potential of this tool to study intracellular processes.
keywords
ION ENERGY-TRANSFER; FLUORESCENT THERMOSENSORS; COORDINATION-COMPOUNDS; GAP ISSUE; THERMOMETRY; SENSORS
subject category
Chemistry; Science & Technology - Other Topics; Materials Science; Physics
authors
Pinol, R; Zeler, J; Brites, CDS; Gu, YY; Tellez, P; Neto, ANC; da Silva, TE; Moreno-Loshuertos, R; Fernandez-Silva, P; Gallego, AI; Martinez-Lostao, L; Martinez, A; Carlos, LD; Millan, A
our authors
Albano Neto Carneiro Neto
Junior Researcher
Carlos Brites
Assistant Professor
Justyna Anna Zeler
Post-Doc Fellowship
Luís Carlos
Full professor
Thiago Estefane da Silva
PhD StudentProjects
Nanoparticles - based 2D thermal bioimaging technologies - NanoTBTech (NanoTBTech)
SusPhotoSolutions - Soluções Fotovoltaicas Sustentáveis (SUSPHOTOSOLUTIONS)
acknowledgements
This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020 and UIDP/50011/2020, financed by Portuguese funds through the FCT/MEC and when appropriate cofinanced by FEDER under the PT2020 Partnership Agreement. Financial support from the project (NanoHeatControl, POCI-01-0145-FEDER-031469), funded by FEDER, through POCI and by Portuguese funds (OE), through FCT/MCTES, by European Union's Horizon 2020 FET Open program under grant agreement nos. 801305 and 829162, Spanish Ministry of Science Innovation and Universities (grant no: PGC2018_095795_B_I00) and Diputacion General de Aragon (E11/17R) are acknowledged. ANCN thanks SusPhotoSolutions project, CENTRO-01-0145FEDER-000005, Portugal, for his grant. The authors acknowledge the use of Servicio General de Apoyo a la InvestigaciOnSAI, Universidad de Zaragoza.