abstract
Ionic liquids (ILs)-based aqueous biphasic systems (ABS) are efficient platforms for the extraction and purification of biomolecules. To find more biocompatible ABS, propylammonium-based ILs, phosphate salts and water were mixed at three temperatures, evaluating the influence of IL and temperature on the liquid-liquid equilibria (LLE). The biphasic region increases with the increase of IL' anionic/cationic chain and decreases with the increase of temperature. There is good agreement between the IL' ability to form ABS and the hydrogen bonding interaction predicted by COSMO-RS. The subtle balance of hydrogen bonding between salt anion-water and IL anion-water governs the phase separation.
keywords
2-PHASE SYSTEMS; PHASE-DIAGRAMS; POLYETHYLENE-GLYCOL; PROTEIN SEPARATION; EXTRACTION; CAROTENOIDS; PARTITION; SOLVENTS; RECOVERY; ABILITY
subject category
Chemistry; Physics
authors
Mussagy, CU; Tabanez, NL; Farias, FO; Kurnia, KA; Mafra, MR; Pereira, JFB
our authors
Jorge Fernando Brandão Pereira
PhD StudentGroups
acknowledgements
This research was supported by grants from the Ministry of Science and Technology, High Education and Technical Vocational Training of Mozambique (MCTESTP, Maputo, Mozambique) through the HEST Project-World Bank and the FAPESP (Sao Paulo Research Foundation Brazil) through the projects, 2014/19793-3, 2014/16424-7, 2015/50058-0. The authors also acknowledge the support from the CAPES-PRINT (Coordination of Superior Level Staff Improvement, Brazil) - Finance Code 88887.367977/2019-00 and Finance Code 001. F. O. Farias acknowledges CAPES and Fundacao Araucaria for the post-doctoral fellowship (88887.354435/2019-00). M. R. Mafra are grateful to the Brazilian National Council for Scientific and Technological Development (CNPq -Grant 310182/2018). The NMR spectrometers are part of the National NMR Network (PTNMR) and are partially supported by Infrastructure Project N degrees 022161 (co-financed by FEDER through COMPETE 2020, POCI and PORL and FCT through PIDDAC) - Portugal.