Lupane-type triterpenoids from Acacia dealbata bark extracted by different methods
authors Rodrigues, VH; de Melo, MMR; Portugal, I; Silva, CM
nationality International
journal INDUSTRIAL CROPS AND PRODUCTS
author keywords Acacia dealbata; GC-MS; Lupenyl acetate; Lupenone; Supercritical fluid extraction; Solid-liquid extraction; Solubility estimation
keywords SUPERCRITICAL-FLUID EXTRACTION; EUCALYPTUS-GLOBULUS BARK; DIOXIDE PLUS ETHANOL; CARBON-DIOXIDE; VOLUMETRIC PROPERTIES; AUSTRALIAN ACACIAS; POLYPHENOLS; OPTIMIZATION; GLUCOSIDES; MIXTURES
abstract The management of invasive species generates large amounts of biomass which end up in low value applications. However, these residues can potentially contain compounds with important bioactive properties. In this study the bark of Acacia dealbata Link (Fabaceae) was extracted by different methods targeting triterpenoids content and bulk product. Batch solid-liquid extraction (SLE-batch), Soxhlet extraction (n-hexane, dichloromethane, ethyl acetate and ethanol) and supercritical fluid extraction (SFE) (CO2 with/without ethanol or ethyl acetate) were performed under different pressure and/or temperature conditions in order to assess total extraction yield (eta(Total)), extract concentrations and yields of lupenyl acetate and lupenone. The extracts composition was analysed by gas chromatography coupled to mass spectroscopy. Summarily: (i) the highest values of eta(Total) were achieved with ethanol by both Soxhlet and SLE-batch, and by SFE at 300 bar, 80 degrees C with 5 wt.% ethanol; (ii) the highest concentrations of triterpenoids were obtained with n-hexane by Soxhlet and SLE-batch and, and by SFE at 200 bar, 40 degrees C with 5 wt.% ethyl acetate; (iii) the maximum individual yields were achieved with ethanol by both Soxhlet and SLE-batch, and by SFE at 200 bar, 40 degrees C with 5 wt.% ethyl acetate. The solubilities of lupenyl acetate, lupenone, tetracosanoic acid and hexacosan-l-ol in the various solvents were theoretically estimated, providing important insights for the interpretation of the experimental results.
publisher ELSEVIER
issn 0926-6690
isbn 1872-633X
year published 2021
volume 170
digital object identifier (doi) 10.1016/j.indcrop.2021.113734
web of science category 12
subject category Agricultural Engineering; Agronomy
unique article identifier WOS:000690448000001

Sponsors

1suponsers_list_ciceco.jpg