resumo
The management of invasive species generates large amounts of biomass which end up in low value applications. However, these residues can potentially contain compounds with important bioactive properties. In this study the bark of Acacia dealbata Link (Fabaceae) was extracted by different methods targeting triterpenoids content and bulk product. Batch solid-liquid extraction (SLE-batch), Soxhlet extraction (n-hexane, dichloromethane, ethyl acetate and ethanol) and supercritical fluid extraction (SFE) (CO2 with/without ethanol or ethyl acetate) were performed under different pressure and/or temperature conditions in order to assess total extraction yield (eta(Total)), extract concentrations and yields of lupenyl acetate and lupenone. The extracts composition was analysed by gas chromatography coupled to mass spectroscopy. Summarily: (i) the highest values of eta(Total) were achieved with ethanol by both Soxhlet and SLE-batch, and by SFE at 300 bar, 80 degrees C with 5 wt.% ethanol; (ii) the highest concentrations of triterpenoids were obtained with n-hexane by Soxhlet and SLE-batch and, and by SFE at 200 bar, 40 degrees C with 5 wt.% ethyl acetate; (iii) the maximum individual yields were achieved with ethanol by both Soxhlet and SLE-batch, and by SFE at 200 bar, 40 degrees C with 5 wt.% ethyl acetate. The solubilities of lupenyl acetate, lupenone, tetracosanoic acid and hexacosan-l-ol in the various solvents were theoretically estimated, providing important insights for the interpretation of the experimental results.
palavras-chave
SUPERCRITICAL-FLUID EXTRACTION; EUCALYPTUS-GLOBULUS BARK; DIOXIDE PLUS ETHANOL; CARBON-DIOXIDE; VOLUMETRIC PROPERTIES; AUSTRALIAN ACACIAS; POLYPHENOLS; OPTIMIZATION; GLUCOSIDES; MIXTURES
categoria
Agricultural Engineering; Agronomy
autores
Rodrigues, VH; de Melo, MMR; Portugal, I; Silva, CM
nossos autores
Carlos Manuel Silva
Professor associado
Inês Portugal
Professor Auxiliar
Marcelo Morais Rodrigues de Melo
Bolseiro de pós-Doutoramento
Vitor Hugo dos Louros Rodrigues
Estudante de doutoramentoProjectos
CICECO - Aveiro Institute of Materials (UIDB/50011/2020)
agradecimentos
This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020 & UIDP/50011/2020, financed by national funds through the FCT/MEC and when appropriate co-financed by FEDER under the PT2020 Partnership Agreement. This work was carried out under the Project inpactus-innovative products and technologies from eucalyptus, Project N.o 21874 funded by Portugal 2020 through European Regional Development Fund (ERDF) in the frame of COMPETE 2020 no246/AXIS II/2017.Authors want to thank the funding from Project AgroForWealth (CENTRO-01-0145-FEDER-000001) , funded by Centro2020, through FEDER and PT2020.