abstract
This work developed a universal UPLC-PDA method based on safe reagents to analyze anthocyanins from different foods. Nine foods were studied by the developed chromatographic method, which was constructed using a solid core C18 column and a binary mobile phase composed of (A) water (0.25 molcitric acid.Lsolvent 1 ), and (B) ethanol. A total running time of 6 min was obtained, the faster comprehensive method for anthocyanins analysis. Mass spectrometry analysis was employed to identify a comprehensive set of 53 anthocyanins comprising glycosylated and acylated cyanidin, pelargonidin, malvidin, peonidin, petunidin, and delphinidin derivatives. Cyanidin-3-O-glucoside (m/z+ 449) and cyanidin-3-O-rutinoside (m/z+ 595) were used as standards to validate the accuracy of the developed method. The analytical parameters were evaluated, including intra-day and inter-day precision, robustness, repeatability, retention factor (k), resolution, and peak symmetry factor. The current method demonstrated excellent chromatographic resolution, making it a powerful tool for analyzing anthocyanins pigments.
keywords
LIQUID-CHROMATOGRAPHY; ANTIOXIDANT ACTIVITY; HPLC-DAD; MS; IDENTIFICATION
subject category
Chemistry; Food Science & Technology; Nutrition & Dietetics
authors
Mesquita, LMD; Contieri, LS; Sanches, VL; Kamikawachi, R; Sosa, FHB; Vilegas, W; Rostagno, MA
our authors
Filipe Hobi Bordon Sosa
Junior Researcher
Leonardo Mendes de Sousa Mesquita
Post-Doc Fellowshipacknowledgements
This work was supported by "Fundacao de Amparo a & nbsp;Pesquisa do Estado de Sao Paulo - FAPESP" through the projects (2018/14582-5 and 2019/13496-0) and fellowships (L.M. de S.M.: 2020/08421-9, 2021/11022-1; L.C.S.: 2020/03623-2, 2021/11023-8). This work was supported by the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, Brazil) (productivity grant 302610/2021-9). Filipe H. B. Sosa acknowledge FCT - Fundacao para a Ciencia e a Tecnologia, I.P. for the researcher contract CEECIND/07209/2022, under the Scientific Employment Stimulus - Individual Call.