Combined theoretical and experimental insights on DNA and BSA binding interactions of Cu(II) and Ni(II) complexes along with the DPPH method of antioxidant assay and cytotoxicity studies

abstract

This present study delineates the syntheses, detailed characterization and anti-proliferative potential against SiHa (cervical cancer cell) of two mononuclear complexes of Cu(ii) and Ni(ii) using a Schiff base ligand (L) derived from 2-hydroxybenzaldehyde and N-methyl-propane 1,3-diamine. The crystallographic results show the centro-symmetric space group of orthorhombic nature (Pccn) for Cu(ii) complex (1) where the central Cu(ii) has an inversion center symmetry with six co-ordinations resulting in a distorted octahedral geometry. Whereas, in complex (2), the two independent Ni(ii) atoms present in the special position within version symmetry and form a distorted geometry of octahedral nature with six coordinations. Absorption spectral titrations with Calf Thymus (CT) DNA and the extent of the decrease in relative emission intensities of DNA-bound ethidium bromide (EB) upon adding the complexes reveal the parallel trend in DNA binding affinities for both the complexes but with a small extent of binding capabilities. Bovine serum albumin (BSA) interaction studies demonstrate that complex 1 exhibits more promiscuous binding with BSA as compared to complex 2 from the spectroscopic and theoretical approaches. alpha,alpha-Diphenyl-beta-picrylhydrazyl (DPPH) free radical scavenging method shows a little antioxidant or free radical scavenging activity for both the studied complexes. Cytotoxicity studies against SiHa expressed that the percentage of cell viability was reduced with time whereas in the same concentration and conditions, the viability percentage was higher for 3T3-L1 (several normal cell lines of mouse). The fluorescence imaging obtained from acridine orange (AO) and ethidium bromide (EtBr) demonstrates that the colour of the cancer cells has changed gradually dictating the cell apoptosis from day 1 to day 3.

keywords

IN-VITRO; COPPER(II) COMPLEXES; RUTHENIUM COMPLEXES; ANTITUMOR-ACTIVITY; CRYSTAL-STRUCTURES; LIGANDS SYNTHESIS; ANTICANCER; MECHANISM; AGENT; CHEMISTRY

subject category

Chemistry

authors

Acharya, P; Kuila, A; Pramanik, U; Hathwar, VR; Brandao, P; Mukherjee, S; Maity, S; Maity, T; Maity, R; Samanta, BC

Groups

G1 - Porous Materials and Nanosystems

Projects

CICECO - Aveiro Institute of Materials (UIDB/50011/2020)

CICECO - Aveiro Institute of Materials (UIDP/50011/2020)

Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)

acknowledgements

Financial assistance from DBT, Govt. of India to Mugberia Gangadhar Mahavidyalaya under Star College Strengthening Scheme (Order No. HRD-11011/161/2020-HRD-DBT, Dated 24/08/2020) is sincerely acknowledged by Dr Bidhan Chandra Samanta. FTIR and Mass spectroscopic data collection and analyses by Dr Debaprasad Mandal, Dept. of Chemistry, IIT, Ropar are gratefully acknowledged here. Kind assistance from Prof. Pralay Maiti, IIT, BHU for cell viability studies is sincerely acknowledged. Prasun Acharya and Arun Kuila wish to thank the authority of Mugberia Gangadhar Mahavidyalaya for having laboratory facilities to carry out the experiments. Ushasi Pramanik would like to acknowledge IISER Bhopal for fellowship. Paula Brandao would like to acknowledge financial assistance of national funds through the FCT/MCTES (PIDDAC) for the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020, UIDP/50011/2020 & LA/P/0006/2020.