abstract
The structure of two different Mg-substituted biphasic (HAP and beta-TCP) mixtures along with the biphasic mixtures without substituted Mg(2+) was investigated using Rietveld refinement technique. The substituted Mg(2+) was found in the beta-TCP phase and its influence on the composition has led to an increase in HAP content of Mg-containing biphasic mixtures when compared with the HAP content detected in pure biphasic mixtures. The refined structural parameters of Ca(10)(PO(4))(6)(OH)(2) and beta-Ca(3)(PO(4))(2) confirmed that all the investigated compositions have crystallized in the corresponding hexagonal (space group P6(3)/m) and rhombohedral (space group R3c) structures. The substitution of lower sized magnesium was found preferentially incorporated at the sixfold-coordinated Ca (5) site of beta-TCP, which is due to the strong Ca (5)center dot O interaction among all the five different Ca sites of beta-Ca(3)(PO(4))(2). The in vitro tests using primary culture of osteoblasts showed that all the tested samples are biocompatible and promising materials for in vivo studies. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 90B: 404-411, 2009
keywords
ALKALINE-PHOSPHATASE; SUBSTITUTED HYDROXYAPATITE; STABILIZING IMPURITY; CRYSTAL-STRUCTURE; SKIN FIBROSIS; PROLIFERATION; REFINEMENT; CERAMICS; BETA-CA3(PO4)2; OSTEOBLASTS
subject category
Engineering; Materials Science
authors
Kannan, S; Goetz-Neunhoeffer, F; Neubauer, J; Rebelo, AHS; Valerio, P; Ferreira, JMF
our authors
José Maria da Fonte Ferreira
Retired Professoracknowledgements
Contract grant sponsor: Portuguese Foundation for Science and Technology contract grant numbers: POCTI/CTM/60207/2004, SFRH/BPD/18737/2004