abstract
Single phase hydroxyapatite (HAp) and biphasic material hydroxyapatite/beta-tricalcium phosphate (HAp/beta-TCP) were obtained from a marine source (Atlantic cod fish bones). Here we report a study on the biological properties of these materials, including cytotoxicity, bioactivity and haemocompatibility. Results showed that the materials are not cytotoxic, neither in their powder nor in pellet form; indeed growth of Saos-2 cells was comparable to that of commercial. The haemolysis rate was lower than 2%; hence the materials can be classified as non-haemolytic. Moreover, when immersed in Simulated Body Fluid (SBF), crystal formation was observed on the surface of both materials. The sintering behaviour of the samples was also studied; both powders showed very high sinterability (density higher than 95% of the theoretical value). Overall, these results confirm the suitability of these materials for biomedical applications. (C) 2015 Elsevier B.V. All rights reserved.
keywords
BETA-TRICALCIUM PHOSPHATE; IN-VITRO; SUBSTITUTED HYDROXYAPATITE; BONE REPAIR; FISH BONES; SURFACE; OSTEOCONDUCTIVITY; EXTRACTION; CERAMICS; BEHAVIOR
subject category
Materials Science
authors
Piccirillo, C; Pullar, RC; Costa, E; Santos-Silva, A; Pintado, MME; Castro, PML
our authors
Rob Pullar
Collaboratoracknowledgements
This work was through the PEst-C/CTM/LA0011/2013 and PEst-OE/EQB/LA0016/2013 programmes, and the iCOD project (Inovadora Tecnologias para a Valorizacao de Subprodutos do Processamento do Bacalhau, contract QREN AdI I vertical bar 466). C. Piccirillo and R.C. Pullar thank FCT for their research grant (SFRH/BPD/86483/2012 and SFRH/BPD/97115/2013, respectively).