abstract
The understanding of the molecular-level interactions between biomolecules and ionic liquids (ILs) in aqueous C media is crucial for the optimization of a number of relevant biotechnological processes. In this work, the influence of a series of amino acids oil the liquid-liquid equilibria between 1-butyl-3-methylimidazolium tricyanomethane and water was studied to evaluate the preferential interactions between these three compounds. The solubility effects observed are dependent oil the polarity, size, and charge distribution of the amino acid side chains and are explained ill terms of a refined version of the model proposed earlier (Freire et al. J. Phys. Chem. B 2009, 113, 202; Tome et al. J. Phys. Chem. B 2009, 113, 2815) for ion specific effects oil aqueous solutions of imidazolium-based ILs. Although acting through different mechanisms, salting-in and salting-out phenomena possess a common basis which is the competition between water-amino acid side chain, IL-amino acid side chain, and water-IL interactions. The delicate balance between these interactions is dependent oil the relative affinities of the biomolecules to water molecules or to IL cation and anion and determines the trend and magnitude of the Solubility effect observed.
keywords
MUTUAL SOLUBILITIES; ENZYMATIC RESOLUTION; BIPHASIC SYSTEMS; 298.15 K; WATER; IMIDAZOLIUM; STABILITY; LIPASE; EXTRACTION; TOXICITY
subject category
Chemistry
authors
Tome, LIN; Dominguez-Perez, M; Claudio, AFM; Freire, MG; Marrucho, IM; Cabeza, O; Coutinho, JAP
our authors
Ana Filipa M. Cláudio
Post-doc Fellowship
Isabel Maria Delgado Jana Marrucho Ferreira
Full professor
Luciana Isabel Nabais Tomé
Post-doc Fellowship
Mara Guadalupe Freire Martins
Coordinating ResearcherGroups
G4 - Renewable Materials and Circular Economy
G5 - Biomimetic, Biological and Living Materials
acknowledgements
The authors thank financial Support from Fundacao para a Ciencia e a Tecnologia for Project PTDC/EQU-FTT/65252/2006 and for post-doctoral grant SFRH/BPD/44926/2008 of Luciana I. N. Tome.