resumo
Helicobacter pylori is the etiologic agent of several gastric disorders. The growing rates of unsuccessfulness of available antibiotics-based therapy led to the need for non-antibiotic compounds for H. pylori treatment. The antibacterial activity of Eucalyptus nitens and Eucalyptus globulus outer bark lipophilic extracts were evaluated against a panel of H. pylori strains, as well as the role of pure triterpenic acids and synthetic mixtures mimicking the natural extracts. Best results were obtained with E. nitens outer bark extract (MIC = 64 mu g/mL) and it was demonstrated that after 6 h of exposure to the MIC, there was a significant decrease ( > 50%) in H. pylori growth. Moreover, it was determined that the antibacterial effect of Eucalyptus spp. outer bark extracts was due to the synergetic effect of four triterpenic acids, namely betulinic, betulonic, ursolic and oleanolic acids. Our findings open new perspectives for development of anti-H. pylori therapies based on the use of these plant extracts.
palavras-chave
PENTACYCLIC TRITERPENES; ANTIMICROBIAL ACTIVITY; PLANT-EXTRACTS; ANTIBACTERIAL ACTIVITY; BIOMASS RESIDUES; NATURAL-PRODUCTS; GASTRIC-CANCER; INFECTION; ACIDS; GLYCOSYLATION
categoria
Agriculture
autores
Parreira, P; Soares, BIG; Freire, CSR; Silvestre, AJD; Reis, CA; Martins, MCL; Duarte, MF
nossos autores
Armando Jorge Domingues Silvestre
Professor Catedrático
Belinda Soares
Estudante de doutoramento
Carmen Sofia da Rocha Freire Barros
Investigador Coordenador
Maria de Fátima Pereira Duarte
Investigador Principalagradecimentos
This work was funded by FEDER funds through the Operational Program Competitiveness Factors (COMPETE), Operational Program for Competitiveness and Internationalization (POCI), Portugal 2020, and national funds by FCT-Foundation for Science and Technology under the project NEucBark-New valorization strategies for Eucalyptus spp. bark extracts (PTDC/AGR-FOR/3187/2012). The authors also acknowledge FCT for the financial support of the Program "Investigador FCT 2012" (C.S.R Freire), CICECO-FCOMP-01-0124-FEDER-037271 and FCTPest-C/CTM/LA0011/2013, Institute for Research and Innovation in Health Sciences (POCI-01-0145FEDER-007274) and UID/AGR/00115/2013 to ICAAM.