resumo
Globally, cancer is one of the leading causes of death, so the development of strategies for an early diagnosis of cancer is of great importance. Biochemical alterations precede morphological changes in cells and tissues, so studying cancer metabolome seems like a reasonable approach for early diagnosis, prognosis and to follow treatment progression. Fourier-Transform Infrared (FTIR) spectroscopy is a valuable tool for studying the metabolome of biological samples, such as cancer cell lines. Unlike staining procedures and other histopathologic approaches, this technique is rapid, non-destructive and does not require reagents. The spectral differences that result from probing the biochemical composition of cancer and normal cells are indicative of distinct metabolic profiles, which allow the discrimination of different cells. Using FTIR spectroscopy and multivariate statistical analysis, several alterations concerning the content of lipids, proteins, nucleic acids and carbohydrates have been identified in cancer cells, some of which can be regarded as potential biomarkers. This review focuses on FTIR spectroscopy as a metabolomics tool to study and characterize cancer cell lines.
palavras-chave
TRANSFORM INFRARED-SPECTROSCOPY; PROSTATE-CANCER; MOLECULAR ALTERATIONS; PROTEIN AGGREGATION; CERVICAL CELLS; HUMAN TISSUES; MUTANT P53; PART I; LINES; METABOLOMICS
categoria
Endocrinology & Metabolism
autores
Santos, F; Magalhaes, S; Henriques, MC; Fardilha, M; Nunes, A
nossos autores
Sandra Vicência Martins Magalhães
Estudante de doutoramentoagradecimentos
This work was supported by grant UID/BIM/04501/2013, co-funded by Fundacao para a Ciencia e Tecnologia I.P. (PIDDAC) and by European Regional Development Fund (FEDER). S.M. is also funded with an individual grant of Fundacao para a Ciencia e Tecnologia I.P. (SFRH/BD/131820/2017).