resumo
It is here reported a new concept based on solvatochromism to distinguish structurally similar compounds in aqueous solutions by the analysis of the stabilization of electronic excited states. The sensitivity of this approach to differentiate similar organic compounds, such as structural isomers or compound differing in the number of methylene groups, or proteins with conformational changes induced by being or not bound to cofactors, differing in two amino acids substitutions, or differing in their glycosylation profile, is demonstrated. The sensitivity of the proposed approach, based on the solvatochromic method, opens the path to its use as an auxiliary analytical tool in biomedical diagnosis/prognosis or in quality control of biologic-based drugs. (C) 2018 Elsevier B.V. All rights reserved.
palavras-chave
ELECTRONIC-SPECTRA; SOLVENT PROPERTIES; GLYCOSYLATION; PROTEINS; SCALE; WATER; BETA
categoria
Chemistry; Physics
autores
Madeira, PP; Loureiro, JA; Freire, MG; Coutinho, JAP
nossos autores
Mara Guadalupe Freire Martins
Investigador Coordenador
Pedro António Palma Madeira
Investigador JúniorGrupos
G4 - Materiais Renováveis e Economia Circular
G5 - Materiais Biomiméticos, Biológicos e Vivos
Projectos
CICECO - Aveiro Institute of Materials (UID/CTM/50011/2013)
Igy Technology: A Purication Platform using Ionic-Liquid-Based Aqueous Biphasic Systems (IGYPURTECH)
agradecimentos
This work was developed in the scope of the project CICECO-Aveiro Institute of Materials (Ref. FCT UID/CTM/50011/2013), financed by national funds through the FCT/MEC and when applicable co-financed by FEDER under the PT2020 Partnership Agreement The authors acknowledge FCT for the post-doctoral fellowship SFRH/BPD/111113/2015 of P.P. Madeira. The authors acknowledge the financial support from the European Union Framework Programme for Research and Innovation HORIZON 2020, under the TEAMING Grant agreement No 739572. The Discoveries CTR. M.G. Freire acknowledges the European Research Council under the European Union's Seventh Framework Programme (FP7/2007-2013)/ERC grant agreement no. 337753. This work was also supported through the project POCI-01-0145-FEDER-006939 funded by the European Regional Development Fund (ERDF), through COMPETE2020 and by national funds, through FCT - Fundacao para a Ciencia e a Tecnologia; and NORTE-01-0145-FEDER-000005 - LEPABE-2 ECO-INNOVATION, supported by North Portugal Regional Operational Program (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (ERDF).