resumo
Nanostructured patches composed of bacterial nanocellulose (BNC), hyaluronic acid (HA) and diclofenac (DCF) were developed, envisioning the treatment of aphthous stomatitis. Freestanding patches were prepared via diffusion of aqueous solutions of HA and DCF, with different concentrations of DCF, into the wet BNC three-dimensional porous network. The resultant dual polysaccharides-based patches with a nanostructured morphology present thermal stability up to 200 degrees C, as well as good dynamic mechanical properties, with a storage modulus higher than 1.0 GPa. In addition, the patches are non-cytotoxic to human keratinocytes (HaCaT cells), with a cell viability of almost 100% after 24 h. The in vitro release profile of DCF from the patches was evaluated in simulated saliva, and the data refer to a diffusion- and swelling-controlled drug-release mechanism. The attained results hint at the possibility of using these dual polysaccharides-based oral mucosal patches to target aphthous stomatitis.
palavras-chave
BACTERIAL CELLULOSE MEMBRANES; TRANSDERMAL DELIVERY; PHYSICAL-PROPERTIES; TOPICAL DICLOFENAC; CONTROLLED-RELEASE; COMPOSITES; DISSOLUTION; EFFICACY; SYSTEMS; GROWTH
categoria
Science & Technology - Other Topics; Materials Science
autores
Carvalho, JPF; Silva, ACQ; Bastos, V; Oliveira, H; Pinto, RJB; Silvestre, AJD; Vilela, C; Freire, CSR
nossos autores
Ana Cristina Quintans da Silva
Investigador
Armando Jorge Domingues Silvestre
Professor Catedrático
Carla Vilela
Professor Auxiliar
Carmen Sofia da Rocha Freire Barros
Investigador Coordenador
Helena Oliveira
Bolseiro de pós-Doutoramento
João Pedro Fernandes Carvalho
Estudante de doutoramento
Ricardo Pinto
Investigadoragradecimentos
This work was developed within the scope of the projects CICECO-Aveiro Institute of Materials (UIDB/50011/2020 and UIDP/50011/2020) and CESAM (UID/AMB/50017/2019), financed by national funds through the Portuguese Foundation for Science and Technology (FCT)/MCTES. The AgroForWealth project (CENTRO-01-0145-FEDER-000001) is acknowledged for the research grant to J.P.F.C. FCT is also acknowledged for the doctoral grant to A.C.Q.S. (SFRH/BD/140230/2018) and for the research contracts under Scientific Employment Stimulus to H.O. (CEECIND/04050/2017) and C.V. (CEECIND/00263/2018). The research contract of V.B. (CDL-CTTRI-161-ARH/2018) is funded by the FCT project (POCI-01-0145-FEDER-031794), while the research contract of R.J.B.P. is funded by national funds (OE), through FCT, in the scope of the framework contract foreseen in the numbers 4, 5 and 6 of article 23, of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19.