abstract
Nanostructured patches composed of bacterial nanocellulose (BNC), hyaluronic acid (HA) and diclofenac (DCF) were developed, envisioning the treatment of aphthous stomatitis. Freestanding patches were prepared via diffusion of aqueous solutions of HA and DCF, with different concentrations of DCF, into the wet BNC three-dimensional porous network. The resultant dual polysaccharides-based patches with a nanostructured morphology present thermal stability up to 200 degrees C, as well as good dynamic mechanical properties, with a storage modulus higher than 1.0 GPa. In addition, the patches are non-cytotoxic to human keratinocytes (HaCaT cells), with a cell viability of almost 100% after 24 h. The in vitro release profile of DCF from the patches was evaluated in simulated saliva, and the data refer to a diffusion- and swelling-controlled drug-release mechanism. The attained results hint at the possibility of using these dual polysaccharides-based oral mucosal patches to target aphthous stomatitis.
keywords
BACTERIAL CELLULOSE MEMBRANES; TRANSDERMAL DELIVERY; PHYSICAL-PROPERTIES; TOPICAL DICLOFENAC; CONTROLLED-RELEASE; COMPOSITES; DISSOLUTION; EFFICACY; SYSTEMS; GROWTH
subject category
Science & Technology - Other Topics; Materials Science
authors
Carvalho, JPF; Silva, ACQ; Bastos, V; Oliveira, H; Pinto, RJB; Silvestre, AJD; Vilela, C; Freire, CSR
our authors
Ana Cristina Quintans da Silva
PhD Student
Armando Jorge Domingues Silvestre
Full professor
Carla Vilela
Principal Researcher
Carmen Sofia da Rocha Freire Barros
Principal Researcher
Helena Oliveira
Post-Doc Fellowship
João Pedro Fernandes Carvalho
PhD Student
Ricardo Pinto
Researcheracknowledgements
This work was developed within the scope of the projects CICECO-Aveiro Institute of Materials (UIDB/50011/2020 and UIDP/50011/2020) and CESAM (UID/AMB/50017/2019), financed by national funds through the Portuguese Foundation for Science and Technology (FCT)/MCTES. The AgroForWealth project (CENTRO-01-0145-FEDER-000001) is acknowledged for the research grant to J.P.F.C. FCT is also acknowledged for the doctoral grant to A.C.Q.S. (SFRH/BD/140230/2018) and for the research contracts under Scientific Employment Stimulus to H.O. (CEECIND/04050/2017) and C.V. (CEECIND/00263/2018). The research contract of V.B. (CDL-CTTRI-161-ARH/2018) is funded by the FCT project (POCI-01-0145-FEDER-031794), while the research contract of R.J.B.P. is funded by national funds (OE), through FCT, in the scope of the framework contract foreseen in the numbers 4, 5 and 6 of article 23, of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19.