Nanocellulose-Based Patches Loaded with Hyaluronic Acid and Diclofenac towards Aphthous Stomatitis Treatment

authors	Carvalho, JPF; Silva, ACQ; Bastos, V; Oliveira, H; Pinto, RJB; Silvestre, AJD; Vilela, C; Freire, CSR
nationality	International
journal	NANOMATERIALS
author keywords	bacterial nanocellulose; hyaluronic acid; diclofenac; nanostructured patches; drug delivery; aphthous stomatitis
keywords	BACTERIAL CELLULOSE MEMBRANES; TRANSDERMAL DELIVERY; PHYSICAL-PROPERTIES; TOPICAL DICLOFENAC; CONTROLLED-RELEASE; COMPOSITES; DISSOLUTION; EFFICACY; SYSTEMS; GROWTH
abstract	Nanostructured patches composed of bacterial nanocellulose (BNC), hyaluronic acid (HA) and diclofenac (DCF) were developed, envisioning the treatment of aphthous stomatitis. Freestanding patches were prepared via diffusion of aqueous solutions of HA and DCF, with different concentrations of DCF, into the wet BNC three-dimensional porous network. The resultant dual polysaccharides-based patches with a nanostructured morphology present thermal stability up to 200 degrees C, as well as good dynamic mechanical properties, with a storage modulus higher than 1.0 GPa. In addition, the patches are non-cytotoxic to human keratinocytes (HaCaT cells), with a cell viability of almost 100% after 24 h. The in vitro release profile of DCF from the patches was evaluated in simulated saliva, and the data refer to a diffusion- and swelling-controlled drug-release mechanism. The attained results hint at the possibility of using these dual polysaccharides-based oral mucosal patches to target aphthous stomatitis.
publisher	MDPI
isbn	2079-4991
year published	2020
volume	10
issue	4
digital object identifier (doi)	10.3390/nano10040628
web of science category	Nanoscience & Nanotechnology; Materials Science, Multidisciplinary
subject category	Science & Technology - Other Topics; Materials Science
unique article identifier	WOS:000539577200031