In vitro immunomodulatory activity of water-soluble glucans from fresh and dried Longan (Dimocarpus longan Lour.)

authors	Lan, HB; Nunes, C; Lopes, GR; Wang, K; Zhao, L; Coimbra, MA; Hu, ZY
nationality	International
journal	CARBOHYDRATE POLYMERS
author keywords	Longan polysaccharides; Glucan; Immunomodulatory activity; Linkage analysis; Cytokines
keywords	IMMUNOSTIMULATORY ACTIVITY; POLYSACCHARIDES; ACTIVATION; ROOT
abstract	Longan (Dimocarpus longan Lour.) is a seasonal tropical fruit used by Chinese medicine in both fresh and dried pulp forms. Their polysaccharides have been reported to have biological activity. However, their composition and immune activity have not yet been disclosed. To fulfil this aim, hot water-soluble polysaccharides of fresh and dried longan pulp were fractionated according to their molecular weight by ultrafiltration (10, 50, 100 kDa cut off). The main polysaccharides recovered were 1,6-linked glucans branched at O-3 (4-8%), O-2 (1%), O-2,4 (0.1%), and O-3,4 (0.1%). The drying process promotes the solubility of the polysaccharides. These glucans from fresh and dried longan pulp have immunomodulatory activity, shown by in vitro phagocytosis, NO, TNF-alpha, and IL6 macrophages production assays. They showed also to inhibit the inflammatory response induced by LPS. The immunological activity of these glucans seems to have different responses dependent on their molecular weight, related to the immune regulatory pathways.
publisher	ELSEVIER SCI LTD
issn	0144-8617
isbn	1879-1344
year published	2021
volume	266
digital object identifier (doi)	10.1016/j.carbpol.2021.118106
web of science category	10
subject category	Chemistry, Applied; Chemistry, Organic; Polymer Science
unique article identifier	WOS:000655699400006