abstract
The present work is an amalgamation of computation and experimental approach to gain an insight into composition-structure-bioactivity relationships of alkali-free bioactive glasses in the CaO-MgO-SiO2-P2O5 system. The glasses have been designed in the diopside (CaO center dot MgO center dot 2SiO(2); Di)-tricalcium phosphate (3CaO center dot P2O5; TCP) binary join by varying the Di/TCP ratio. The melt-quenched glasses have been investigated for their structure by molecular dynamic (MD) simulations as well as by nuclear magnetic resonance spectroscopy (NMR). In all the investigated glasses silicate and phosphate components are dominated by Q(2) (Si) and Q(0) (P) species, respectively. The apatite forming ability of the glasses was investigated using X-ray diffraction (XRD), infrared spectroscopy after immersion of glass powders in simulated body fluid (SBF) for time durations varying between 1 h and 14 days, while their chemical degradation has been studied in Tris-HCl in accordance with ISO 10993-14. All the investigated glasses showed good bioactivity without any substantial variation. A significant statistical increase in metabolic activity of human mesenchymal stem cells (hMSCs) when compared to the control was observed for Di-60 and Di-70 glass compositions under both basal and osteogenic conditions. (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
keywords
LIME-PHOSPHOSILICATE GLASSES; BETA-TRICALCIUM PHOSPHATE; STRUCTURE-PROPERTY RELATIONSHIPS; MESENCHYMAL STEM-CELLS; MARROW STROMAL CELLS; IN-VITRO; MOLECULAR-DYNAMICS; SILICATE-GLASSES; STRONTIUM SUBSTITUTION; COMPOSITION DEPENDENCE
subject category
Engineering; Materials Science
authors
Kapoor, S; Semitela, A; Goel, A; Xiang, Y; Du, JC; Lourenco, AH; Sousa, DM; Granja, PL; Ferreira, JMF
our authors
José Maria da Fonte Ferreira
Retired Professor
Saurabh Kapoor
PhD Studentacknowledgements
The supports from CICECO, University of Aveiro and from Foundation for Science and Technology (FCT), Portugal, in the frame of the Project Reference PTDC/CTM/99489/2008 are acknowledged. JD acknowledges financial support from NSF DMR Ceramic program (project # 1105219). This work was partially financed by European Regional Development Fund (ERDF) through the Programa Operacional Factores de Competitividade - COMPETE, by Portuguese funds through FCT in the framework of the project PEst-C/SAU/LA0002/2011, and co-financed by North Portugal Regional Operational Programme (ON.2 - O Novo Norte) in the framework of project SAESCTN-PIIC&DT/2011, under the National Strategic Reference Framework (NSRF). The authors also thank Dr. Mattias Eden Arrhenius Laboratory, Stockholm University, Sweden, for fruitful discussions regarding the NMR results.