abstract
Obesity is a public health problem and a risk factor for pathologies such type 2 diabetes mellitus, cardiovascular diseases, and nonalcoholic fatty liver disease. Given these clinical implications, there is a growing interest to understand the pathophysiological mechanism of obesity. Changes in lipid metabolism have been associated with obesity and obesity-related complications. However, changes in the lipid profile of obese children have been overlooked. In the present work, we analyzed the serum phospholipidome of overweight and obese children by HILIC-MS/MS and GC-MS. Using this approach, we have identified 165 lipid species belonging to the classes PC, PE, PS, PG, PI, LPC, and SM. The phospholipidome of overweight (OW) and obese (OB) children was significantly different from normal-weight children (control). Main differences were observed in the PI class that was less abundant in OW and OB children and some PS, PE, SM, and PC lipid species are upregulated in obese and overweight children. Although further studies are needed to clarify some association between phospholipid alterations and metabolic changes, our results highlight the alteration that occurs in the serum phospholipid profile in obesity in children.
keywords
POLYUNSATURATED FATTY-ACIDS; PLASMA PHOSPHOLIPIDS; INDUCED INFLAMMATION; INSULIN-RESISTANCE; METABOLIC SYNDROME; CHILDHOOD OBESITY; PROFILE; LIPIDS; DISEASE; ADIPOSE
subject category
Biochemistry & Molecular Biology
authors
Anjos, S; Feiteira, E; Cerveira, F; Melo, T; Reboredo, A; Colombo, S; Dantas, R; Costa, E; Moreira, A; Santos, S; Campos, A; Ferreira, R; Domingues, P; Domingue, MRM
our authors
acknowledgements
Thanks are due for the financial support to QOPNA (FCT UID/QUI/00062/2019) and CESAM (UID/AMB/50017/2019), and Portuguese Mass Spectrometry Network (LISBOA-01-0145-FEDER-402-022125) CICECO-Aveiro Institute of Materials (UID/CTM/50011/2019) to FCT/MCTES through national funds (PIDDAC), and the cofunding by the FEDER, within the PT2020 Partnership Agreement and Compete 2020. T.M. (BPD/UI51/5388/2017) is grateful to FCT for her grant. Simone Colombo grant was funded by the European Commission's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement number 675132 (MSCA-ITN-ETN MASSTR-PLAN).