Extraction and Physicochemical Characterization of Chitin from Cicada orni Sloughs of the South-Eastern French Mediterranean Basin


Chitin is a structural polysaccharide of the cell walls of fungi and exoskeletons of insects and crustaceans. In this study, chitin was extracted, for the first time in our knowledge, from the Cicadaorni sloughs of the south-eastern French Mediterranean basin by treatment with 1 M HCl for demineralization, 1 M NaOH for deproteinization, and 1% NaClO for decolorization. The different steps of extraction were investigated by Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Thermogravimetric Analysis (TGA), and Scanning Electron Microscopy (SEM). Results demonstrated that the extraction process was efficiently performed and that Cicadaorni sloughs of the south-eastern French Mediterranean basin have a high content of chitin (42.8%) in the alpha -form with a high degree of acetylation of 96% +/- 3.4%. These results make Cicadaorni of the south-eastern French Mediterranean basin a new and promising source of chitin. Furthermore, we showed that each step of the extraction present specific characteristics (for example FTIR and XRD spectra and, consequently, distinct absorbance peaks and values of crystallinity as well as defined values of maximum degradation temperatures identifiable by TGA analysis) that could be used to verify the effectiveness of the treatments, and could be favorably compared with other natural chitin sources.



subject category

Biochemistry & Molecular Biology; Chemistry


Poerio, A; Petit, C; Jehl, JP; Arab-Tehrany, E; Mano, JF; Cleymand, F

our authors


This work was supported partly by the French PIA project Lorraine Universite d'excellence, reference ANR-15-IDEX-04-LUE. This work has been also supported by CNRS GDR 2088 BIOMIM.

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