Metabolic composition of the cyanobacterium Nostoc muscorum as a function of culture time: A 1H NMR metabolomics study


Cyanobacteria are considered a factory of added-value compounds. However, knowledge about the array of interesting compounds that could be extracted from these prokaryotic organisms is still very limited. Nuclear Magnetic Resonance (NMR) spectroscopy is a widely used technique for metabolic profiling that allows an overview of the main metabolites present in complex biological matrices. In this work, high resolution 1H NMR was employed to screen the metabolic composition of the freshwater cyanobacterium Nostoc muscorum. This species showed high longevity, being able to grow for more than one hundred days without any medium sup-plementation. During the period of study, several interesting metabolites were detected, such as several sugars and oligosaccharides, lipids (e.g., glycolipids, omega-3 and omega-6 fatty acids), amino acids, including mycosporin-like, peptides, and pigments (e.g., chlorophyll a and carotenoids). Owing to the long-term monitoring implemented in this study, the production of these compounds could be associated to specific moments of the growth of N. muscorum, providing new insights into the most appropriate harvesting time points for the biotechnological exploitation of specific molecules



subject category

Biotechnology & Applied Microbiology


Macario, IPE; Veloso, T; Romao, J; Goncalves, FJM; Pereira, JL; Duarte, IF; Ventura, SPM

our authors


This work was developed within the scope of the projects CICECO- Aveiro Institute of Materials, (UIDB/50011/2020, UIDP/50011/2020 & LA/P/0006/2020) and CESAM-Centre for Environmental and Marine Studies (UIDB/50017/2020 & UIDP/50017/2020 & LA/P/0094/2020), financed by national funds through the FCT/MEC (PIDDAC). The study was supported by the project REFINECYANO, funded by FCT (PTDC/ BTA-BTA/30914/2017). I.P.E. Macario and T. Veloso are recipients of individual research grants by FCT financed by the FCT/MEC and co- financed by FEDER program (SFRH/BD/123850/2016 and SFRH/BD/ 147346/2019, respectively). The NMR spectrometer is part of the National NMR Network (PTNMR), partially supported by Infrastructure Project N? 022161 (co-financed by FEDER through COMPETE 2020, POCI and PORL and FCT through PIDDAC).

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