abstract
Significant advancements in various research and technological fields have contributed to remarkable findings on the physiological dynamics of the human body. Tomore closely mimic the complex physiological environment, research has moved from two-dimensional (2D) culture systems to more sophisticated three-dimensional (3D) dynamic cultures. Unlike bioreactors or microfluidic-based culture models, cells are typically seeded on polymeric substrates or incorporated into 3D constructs which are mechanically stimulated to investigate cell response to mechanical stresses, such as tensile or compressive. This review focuses on the working principles of mechanical stimulation devices currently available on the market or custom-built by research groups or protected by patents and highlights the main features still open to improvement. These are the features which could be focused on to perform, in the future, more reliable and accurate mechanobiology studies. [GRAPHICS] .
keywords
MESENCHYMAL STEM-CELLS; MARROW STROMAL CELLS; CYCLIC TENSILE STRAIN; ON-A-CHIP; OSTEOGENIC DIFFERENTIATION; IN-VITRO; PROGENITOR CELLS; GENE-EXPRESSION; MATRIX; COMPRESSION
subject category
Engineering
authors
Melo-Fonseca, F; Carvalho, O; Gasik, M; Miranda, G; Silva, FS
our authors
Georgina Miranda
Assistant ProfessorProjects
CICECO - Aveiro Institute of Materials (UIDB/50011/2020)
Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)
acknowledgements
This work was supported by FCT (Fundacao para a Ciencia e a Tecnologia) through the grant SFRH/BD/141056/2018, the project PTDC/EME-EME/1442/2020 and under the national support to R&D units grant, through the reference projects UIDB/04436/2020 and UIDP/04436/2020. In addition, this work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020, UIDP/50011/2020& LA/P/0006/2020, financed by national funds through the FCT/MEC (PIDDAC).