abstract
Galectin-3 (Gal-3) is a carbohydrate-binding protein associated with the development and progress of heart failure. Here, we report the first colorimetric and low-cost approach for detecting and quantifying Gal-3 using gold nanoparticles (AuNPs) bioconjugated with Gal-3 antibody. The interaction of Gal-3 with the resulting nanoprobes led to a linear response of the absorbance ratio A(750nm)/A(526nm) to Gal-3 concentration, accompanied by a change in color intensity. The assay showed a linear optical response even in complex samples, such as saliva and fetal bovine serum (FBS), up to a concentration of 200 mu g L-1. The limit of detection (LOD) followed the trend LODPBS (10.0 mu g L-1) < LODsaliva (22.6 mu g L-1) < LODFBS (25.3 mu g L-1). The potential applicability of this method to the analysis of human plasma samples was also demonstrated. Compared to conventional detection techniques, this colorimetric assay provides faster results (similar to 1 h) and is more cost-effective due to the use of simple and unexpensive equipment. This assay represents an exciting solution for the rapid screening of high risk for rapid progression of heart failure in patient samples (Gal-3 > 25.9 mu g L-1).
keywords
EXPRESSION; SINGLE
subject category
Chemistry; Food Science & Technology; Spectroscopy
authors
António, M; Lima, T; Ferreira, R; Fardilha, M; Bastos, JM; Vitorino, R; Daniel-da-Silva, AL
our authors
Ana Luísa Daniel da Silva
Principal Researcher
Maria de Almeida Vasconcelos António
PhD StudentProjects
CICECO - Aveiro Institute of Materials (UIDB/50011/2020)
CICECO - Aveiro Institute of Materials (UIDP/50011/2020)
Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)
Projeto de Investigação Exploratória: Ana Luísa Silva (IF/00405/2014)
Other
Cover
Mediaacknowledgements
This work was supported by the Portuguese Foundation for Science and Technology (FCT), FCT/MEC (PIDDAC), European Union, QREN, FEDER, and COMPETE for the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020 & UIDP/50011/2020 & LA/P/0006/2020, the project iBiMED (UIDB/04501/2020, POCI-01-0145-FEDER-007628), UnIC (UID/IC/00051/2019) and the project LAQV- REQUIMTE (UIDB/50006/2020). M. A. thanks FCT for the PhD grants (SFRH/BD/136881/2018; COVID/BD/152911/2023). T. L. thanks FCT for the PhD grant (SFRH/BD/136904/2018). A. L. D.-d.-S. acknowledges FCT for the contract (IF/00405/2014). R. V. thanks the FCT for the Investigator Grant (IF/00286/2015). COST Action CA21153Network for implementing multiomics approaches in atherosclerotic cardiovascular disease prevention and research. The graphical abstract was built using images from Servier Medical Art. Servier Medical Art by Servier is licensed under a Creative Commons Attribution 3.0 Unported License (https://creativecommons.org/licenses/by/3.0/).