High-resolution H-1 homonuclear dipolar recoupling NMR spectra of biological solids at MAS rates up to 67 kHz

abstract

Two-dimensional H-1 homonuclear correlation NMR spectra of solids of biological interest have been recorded at high magnetic fields (14.1 and 18.8 T) and MAS rates up to 67 kHz, using RNnv symmetry-based homonuclear recoupling and CRAMPS decoupling; this method affords exceptional spectral resolution and is well suited to probe H-1-H-1 proximities in powdered solids. (C) 2009 Elsevier Inc. All rights reserved.

keywords

NUCLEAR-MAGNETIC-RESONANCE; ANGLE-SPINNING NMR; STATE NMR; PULSE SEQUENCES; MULTIPLE-PULSE; SPECTROSCOPY; FIELD; EXCITATION; RECOVERY

subject category

Biochemistry & Molecular Biology; Physics; Spectroscopy

authors

Mafra, L; Siegel, R; Fernandez, C; Schneider, D; Aussenac, F; Rocha, J

our authors

acknowledgements

We thank FCT, FEDER and POCTI for financial support and post-doc grant (RS). The Portuguese NMR Network is acknowledge for granting access to the 18.8 T NMR Bruker spectrometer at ITQB. We thank Dr. Pedro Lamosa for assistance on the spectrometer.

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