abstract
Composites filled with a silicate glass (CSi) and a new borate glass (CB) were developed and compared in terms of their in vitro behaviour both in acellular and cellular media. Acellular tests were carried out in SBF and the composites were characterized by SEM-EDS, XRD and ICP. Biocompatibility studies were investigated by in vitro cell culture with MG-63 osteoblast-like and human bone marrow cells. The growth of spherical calcium phosphate aggregates was observed in acellular medium on all composite surfaces indicating that these materials became potentially bioactive. The biological assessment resulted in a dissimilar behavior of the composites. The CSi demonstrated an inductive effect on the proliferation of cells. The cells showed a normal morphology and high growth rate when compared to standard culture plates. Contrarily, inhibition of cell proliferation occurred in the CB probably due to its high degradation rate, leading to high B and Mg ionic concentration in the cell culture medium.
keywords
IN-VITRO BIOACTIVITY; SOL-GEL GLASSES; BONE CELL-CULTURES; OSTEOBLASTIC DIFFERENTIATION; MECHANICAL-PROPERTIES; PHOSPHATE; HYDROXYAPATITE; PROLIFERATION; APATITE; GROWTH
subject category
Engineering; Materials Science
authors
Lopes, PP; Ferreira, BJML; Gomes, PS; Correia, RN; Fernandes, MH; Fernandes, MHV
our authors
Bárbara Joana Martins Leite Ferreira
Junior Researcher
Maria Helena Figueira Vaz Fernandes
Retired Professoracknowledgements
The authors acknowledge the Portuguese Foundation for Science and Technology (FCT) and the Center for Research in Ceramic and Composite Materials, CICECO, at the University of Aveiro for financial support.