resumo
A new range of Goods buffer ionic liquids (GB-ILs), displaying simultaneously the properties of ionic liquids and Goods buffers, were synthesized by a combination of Goods buffer anions (MOPSO, BES, TAPSO, and CAPSO) and tetrabutylammonium, tetrabutylphosphonium, and cholinium cations via an acidbase neutralization reaction. The activity and stability of a lipolytic enzyme from Pseudomonas cepacia in aqueous solutions of these buffers were evaluated, and the results show their advantage as media for enzymatic reactions when compared to conventional phosphate buffers. Moreover aqueous biphasic systems (ABS) composed by these GB-ILs and potassium citrate were investigated and shown to be highly effective and selective for the partitioning of the lipolytic enzyme into the GB-IL-rich phase. The results allow for the development of an efficient and biocompatible process combining the self-buffering and enzyme-stabilizing properties of the GB-ILs in the reaction step, with the advantages of GB-ILs as extraction solvents in ABS.
palavras-chave
AQUEOUS BIPHASIC SYSTEMS; FISCHERI MARINE-BACTERIA; BOVINE SERUM-ALBUMIN; ENHANCED EXTRACTION; BIOLOGICAL-RESEARCH; AQUATIC ORGANISMS; GOODS BUFFERS; LIPASE; TOXICITY; PURIFICATION
categoria
Chemistry; Science & Technology - Other Topics; Engineering
autores
Lee, SY; Vicente, FA; Silva, FAE; Sintra, TE; Taha, M; Khoiroh, I; Coutinho, JAP; Show, PL; Ventura, SPM
nossos autores
Ana Francisca Osório de Almeida Coelho e Silva
Investigador Júnior
Filipa Alexandra André Vicente
Estudante de doutoramento
Mohamed Taha Ahmed Amin
Bolseiro de pós-Doutoramento
Sónia Patrícia Marques Ventura
Professor Auxiliar
Tânia Ereira Sintra
Bolseiro de pós-DoutoramentoGrupos
G4 - Materiais Renováveis e Economia Circular
G5 - Materiais Biomiméticos, Biológicos e Vivos
agradecimentos
We gratefully acknowledge the financial support from the Malaysia Toray Science Foundation, Fundamental Research Grant Scheme ref. FRGS/1/2015/SGOS/UNIM/03/1, Ministry of Science, Technology and Innovation of Malaysia ref. 02-02-12-SF0256 and MyBrain 15 Scholarship for L.S.Y. from Ministry of Higher Education of Malaysia. We are grateful for the financial support from FEDER funds through the program COMPETE and for national funds through the Portuguese Foundation for Science and Technology (FCT) for CICECO-Aveiro Institute of Materials ref. FCT UID/CTM/50011/2013, the research project PTDC/ATP-EAM/5331/2014, and the doctoral grants SFRH/BD/94901/2013, SERH/BD/85871/2012, SFRH/BD/101683/2014 for F.A.e.S., T.E.S., and F.A.V., and the postdoctoral grant SFRH/BPD/79263/2011 for S.P.M.V.