resumo
Gold nanoparticles (AuNPs) are one of the most studied nanosystems with great potential for biomedical applications, including cancer therapy. Although some gold-based systems have been described, the use of green and faster methods that allow the control of their properties is of prime importance. Thus, the present study reports a one-minute microwave-assisted synthesis of fucoidan-coated AuNPs with controllable size and high antitumoral activity. The NPs were synthesized using a fucoidan-enriched fraction extracted from Fucus vesiculosus, as the reducing and capping agent. The ensuing monodispersed and spherical NPs exhibit tiny diameters between 5.8 and 13.4 nm for concentrations of fucoidan between 0.5 and 0.05% (w/v), respectively, as excellent colloidal stability in distinct solutions and culture media. Furthermore, the NPs present antitumoral activity against three human tumor cell lines (MNT-1, HepG2, and MG-63), and flow cytometry in combination with dark-field imaging confirmed the cellular uptake of NPs by MG-63 cell line.
palavras-chave
SULFATED POLYSACCHARIDES; NANOPARTICLES; NANOMATERIALS; EXTRACTION; PROGRESS
categoria
Materials Science
autores
Pinto, RJB; Bispo, D; Vilela, C; Botas, AMP; Ferreira, RAS; Menezes, AC; Campos, F; Oliveira, H; Abreu, MH; Santos, SAO; Freire, CSR
nossos autores
Alexandre Manuel Pedroso Botas
Bolseiro de pós-Doutoramento
Carla Vilela
Professor Auxiliar
Carmen Sofia da Rocha Freire Barros
Investigador Coordenador
Daniela Bispo
Estudante de doutoramento
Helena Oliveira
Bolseiro de pós-Doutoramento
Maria Rute de Amorim e Sá Ferreira André
Professor Catedrático
Ricardo Pinto
Investigador
Sónia Andreia Oliveira Santos
Investigador AuxiliarGrupos
G2 - Materiais Fotónicos, Eletrónicos e Magnéticos
G3 - Materiais Eletroquímicos, Interfaces e Revestimentos
G4 - Materiais Renováveis e Economia Circular
G5 - Materiais Biomiméticos, Biológicos e Vivos
agradecimentos
This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020 & UIDP/50011/2020, and CESAM, UID/AMB/50017/2019, financed by national funds through the FCT/MEC and when appropriate co-financed by FEDER under the PT2020 Partnership Agreement. The research contract of R.J.B. Pinto is funded by national funds (OE), through FCT-Fundacao para a Ciencia e a Tecnologia, I.P., in the scope of the framework contract foreseen in the numbers 4, 5 and 6 of the article 23, of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19. The research contract of S.A.O. Santos is funded by the project AgroForWealth (CENTRO-01-0145-FEDER-000001). FCT is also acknowledged for the research contracts under Scientific Employment Stimulus to C. Vilela (CEECIND/00263/2018), H. Oliveira (CEECIND/04050/2017) and C.S.R. Freire (CEECIND/00464/2017). A.M.P. Botas acknowledges SusPhotoSolutions (CENTRO-01-0145-FEDER-000005).