One-Minute Synthesis of Size-Controlled Fucoidan-Gold Nanosystems: Antitumoral Activity and Dark Field Imaging


Gold nanoparticles (AuNPs) are one of the most studied nanosystems with great potential for biomedical applications, including cancer therapy. Although some gold-based systems have been described, the use of green and faster methods that allow the control of their properties is of prime importance. Thus, the present study reports a one-minute microwave-assisted synthesis of fucoidan-coated AuNPs with controllable size and high antitumoral activity. The NPs were synthesized using a fucoidan-enriched fraction extracted from Fucus vesiculosus, as the reducing and capping agent. The ensuing monodispersed and spherical NPs exhibit tiny diameters between 5.8 and 13.4 nm for concentrations of fucoidan between 0.5 and 0.05% (w/v), respectively, as excellent colloidal stability in distinct solutions and culture media. Furthermore, the NPs present antitumoral activity against three human tumor cell lines (MNT-1, HepG2, and MG-63), and flow cytometry in combination with dark-field imaging confirmed the cellular uptake of NPs by MG-63 cell line.




Materials Science


Pinto, RJB; Bispo, D; Vilela, C; Botas, AMP; Ferreira, RAS; Menezes, AC; Campos, F; Oliveira, H; Abreu, MH; Santos, SAO; Freire, CSR

nossos autores


This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020 & UIDP/50011/2020, and CESAM, UID/AMB/50017/2019, financed by national funds through the FCT/MEC and when appropriate co-financed by FEDER under the PT2020 Partnership Agreement. The research contract of R.J.B. Pinto is funded by national funds (OE), through FCT-Fundacao para a Ciencia e a Tecnologia, I.P., in the scope of the framework contract foreseen in the numbers 4, 5 and 6 of the article 23, of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19. The research contract of S.A.O. Santos is funded by the project AgroForWealth (CENTRO-01-0145-FEDER-000001). FCT is also acknowledged for the research contracts under Scientific Employment Stimulus to C. Vilela (CEECIND/00263/2018), H. Oliveira (CEECIND/04050/2017) and C.S.R. Freire (CEECIND/00464/2017). A.M.P. Botas acknowledges SusPhotoSolutions (CENTRO-01-0145-FEDER-000005).

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