resumo
Microalgae, macroalgae and cyanobacteria are photosynthetic microorganisms, prokaryotic or eukaryotic, living in saline or freshwater environments. These have been recognized as valuable carbon sources, able to be used for food, feed, chemicals, and biopharmaceuticals. From the range of valuable compounds produced by these cells, some of the most interesting are the pigments, including chlorophylls, carotenoids, and phycobiliproteins. Phycobiliproteins are photosynthetic light-harvesting and water-soluble proteins. In this work, the downstream processes being applied to recover fluorescent proteins from marine and freshwater biomass are reviewed. The various types of biomasses, namely macroalgae, microalgae, and cyanobacteria, are highlighted and the solvents and techniques applied in the extraction and purification of the fluorescent proteins, as well as their main applications while being fluorescent/luminescent are discussed. In the end, a critical perspective on how the phycobiliproteins business may benefit from the development of cost-effective downstream processes and their integration with the final application demands, namely regarding their stability, will be provided.
palavras-chave
AQUEOUS 2-PHASE SYSTEMS; ULTRASOUND-ASSISTED EXTRACTION; ONE-STEP PURIFICATION; R-PHYCOERYTHRIN; B-PHYCOERYTHRIN; C-PHYCOCYANIN; CARBON-DOTS; SPIRULINA-PLATENSIS; PALMARIA-PALMATA; GRATELOUPIA-TURUTURU
categoria
Chemistry
autores
Kovaleski, G; Kholany, M; Dias, LMS; Correia, SFH; Ferreira, RAS; Coutinho, JAP; Ventura, SPM
nossos autores
Maria Rute de Amorim e Sá Ferreira André
Professor Catedrático
Mariam Kholany
Colaborador
Sónia Patrícia Marques Ventura
Professor AuxiliarGrupos
G2 - Materiais Fotónicos, Eletrónicos e Magnéticos
G4 - Materiais Renováveis e Economia Circular
Projectos
CICECO - Aveiro Institute of Materials (UIDB/50011/2020)
CICECO - Aveiro Institute of Materials (UIDP/50011/2020)
Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)
Solar-Powered Smart Windows for Sustainable Buildings (SOLPOWINS)
agradecimentos
This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020, UIDP/50011/2020 and LA/P/0006/2020, and Instituto de Telecomunicacoes, UIDB/50008/2020-UIDP/50008/2020 and the projects SOLPOWINS (PTDC/CTM-REF/4304/2020) and PLANETa (CENTRO-01-0247-FEDER-181242) financed by national funds through the FCT/MEC (PIDDAC). S. F. H. C. thanks European Space Agency (ESA STAR AO 2-1790/21/NL/GLC/ov). GK acknowledges the financial support through BD/REITORIA/9328/2020, MK and LD thank FCT for the financial support through SFRH/BD/138413/2018 and UI/BD/153491/2022, respectively. The authors also thank the financial support from FCT considering the following references: UI/BD/153491/2022, LD UI/BD/153491/2022, and PTDC/BTA-BTA/30914/2017.