abstract
There is an increasing concern on the consequences of the presence of micro(nano)plastics to marine organisms. The present study aimed to provide information on the effects of polystyrene nanoplastics (PSNPs) to fish cells alone and combined with human pharmaceuticals, other emerging contaminants, using as biological models marine fish cell lines SAF-1 and DLB-1. Cells were exposed for 24 h to 100 tun PSNPs, starting at 0.001 up to 10 mg/L, to assess effects on viability and activity of catalase (antioxidant defense) and glutathione S-trans-ferases (phase II biotransformation and antioxidant defense). The viability of cells was also evaluated after exposure to human pharmaceuticals alone and combined with PSNPs. Overall, PSNPs failed to be cytotoxic but data proved their ability to alter the toxicity of human pharmaceuticals. DLB-1 was the most sensitive cell line to PSNPs. Data support the use of marine fish cell lines in the study of the effects of micro(nano)plastics.
keywords
OXIDATIVE STRESS; MICROPLASTIC INGESTION; SILICA NANOPARTICLES; HEAD-KIDNEY; ORGANISMS; TOXICITY; AURATA; GEMFIBROZIL; ADSORPTION; TRANSPORT
subject category
Environmental Sciences & Ecology; Pharmacology & Pharmacy; Toxicology
authors
Almeida, M; Martins, MA; Soares, AMV; Cuesta, A; Oliveira, M
our authors
Manuel António Martins da Silva
Researcheracknowledgements
Thanks are due for the financial support to CESAM (UID/AMB/50017/2019), to FCT/MCTES through national funds, and the co-funding by the FEDER, within the PT2020 Partnership Agreement and Compete 2020. MO had financial support of the program Investigador FCT (IF/00335/2015), co-funded by the Human Potential Operational Program and European Social Fund.