Using aqueous solutions of ionic liquids as chlorophyll eluents in solid-phase extraction processes


There is a need for handy and fast techniques to purify biomolecules, increasing their stability and value, because the separation units are current bottlenecks in downstream processes. Solid-phase extraction is a technique that enables the purification of a compound by its adsorption from a liquid matrix. The AmberLiteTM HPR900 OH resin allows the separation of chlorophylls from complex extracts, however the recovery of the chlorophylls is not easy to achieve. An innovative procedure to elute the chlorophyll from AmberLiteTM HPR900 OH, based on the use of aqueous solutions of surface-active ionic liquids is proposed in this work. The operational conditions were optimized, showing that the resin can be reused for at least five cycles without losing its efficiency and the chemical structures of the pigments recovered were identified.






Vaz, BMC; Martins, M; Mesquita, LMD; Neves, MC; Fernandes, APM; Pinto, DCGA; Neves, MGPMS; Coutinho, JAP; Ventura, SPM

nossos autores


This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020 & UIDP/50011/2020, financed by national funds through the Portuguese Foundation for Science and Technology/MCTES. Thanks are also due to the University of Aveiro and FCT/MCT for the financial support of LAQV-REQUIMTE (UIDB/50006/2020). Margarida Martins thanks Fundacao para a Ciencia e a Tecnologia (FCT) for the PhD grant (SFRH/BD/122220/2016). Marcia C. Neves acknowledges FCT, I.P. for the research contract CEE-CIND/00383/2017 under the CEEC Individual 2017. The NMR spectrometers are part of the National NMR Network (PTNMR) and are partially supported by Infrastructure Project N. 022161 (co-financed by FEDER through COMPETE 2020, POCI and PORL and FCT through PIDDAC).

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