Hydroperoxide production from linoleic acid by heterologous Gaeumannomyces graminis tritici lipoxygenase: Optimization and scale-up
authors Villaverde, JJ; van der Vlist, V; Santos, SAO; Haarmann, T; Langfelder, K; Pirttimaa, M; Nyyssola, A; Jylha, S; Tamminen, T; Kruus, K; de Graaff, L; Neto, CP; Simoes, MMQ; Domingues, MRM; Silvestre, AJD; Eidner, J; Buchert, J
nationality International
journal CHEMICAL ENGINEERING JOURNAL
author keywords Lipoxygenase; Linoleic acid; Fatty acid hydroperoxides; Optimization; Scale-up
keywords UNSATURATED FATTY-ACIDS; SOYBEAN LIPOXYGENASE; IMMOBILIZED LIPOXYGENASE; MANGANESE LIPOXYGENASE; MASS-SPECTROMETRY; OXIDATION; IDENTIFICATION; SYSTEM; STATE; OIL
abstract Linoleic acid was converted into hydroperoxides by a Gaeumannomyces graminis tritici lipoxygenase produced recombinantly in Trichoderma reesei. Hydroperoxide production was optimized using a face-centred experimental design in order to study the effects of pH, temperature and time on the conversion of linoleic acid into four regioisomeric hydroperoxyoctadecadienoic acids (HPODE): 13-(Z,E)-, 9-(E,Z)-, 13-(E,E)-, 9-(E,E)-HPODE. Fitting equations described satisfactorily the system behavior and showed that reaction time was the most influencing independent variable. A set of independent variables (pH = 6.7, temperature = 23.9 degrees C and time = 18 h) allowed to obtain high yields of hydroperoxides (88.0%) with a good selectivity for the 13-(Z,E)-HPODE isomer (47.4%) when the initial substrate concentration was 10 g/L. The production was further investigated using industrially relevant linoleic acid concentrations (100-300 g/L) leading to HPODE yields of similar to 40% and the volumetric productivity 3.6 g/(L h), and a selectivity for 13-(Z,E)-HPODE of around 74%. (c) 2012 Elsevier B.V. All rights reserved.
publisher ELSEVIER SCIENCE SA
issn 1385-8947
year published 2013
volume 217
beginning page 82
ending page 90
digital object identifier (doi) 10.1016/j.cej.2012.11.090
web of science category Engineering, Environmental; Engineering, Chemical
subject category Engineering
unique article identifier WOS:000316835600011
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