Sequential recovery of C-phycocyanin and chlorophylls from Anabaena cylindrica

authors	Sintra, TE; Bagagem, SS; Ahsaie, FG; Fernandes, A; Martins, M; Macario, IPE; Pereira, JL; Goncalves, FJM; Pazuki, G; Coutinho, JAP; Ventura, SPM
nationality	International
journal	SEPARATION AND PURIFICATION TECHNOLOGY
author keywords	Cyanobacteria; Anabaena cylindrica; C-phycocyanin; Chlorophylls; Sequential extraction; Purification; Aqueous biphasic systems; Stability
keywords	AQUEOUS 2-PHASE SYSTEMS; IONIC-LIQUID; MEDIATED EXTRACTION; BIPHASIC SYSTEMS; EFFICIENT METHOD; BLOCK-COPOLYMER; DRY BIOMASS; PURIFICATION; SEPARATION; OPTIMIZATION
abstract	Cyanobacteria are attracting the attention worldwide as a reliable and sustainable feedstock for the production of biofuels, food colorants and biochemically active compounds. Anabaena cylindrica (A. cylindrica) is a cyanobacterium with a significant widespread occurrence in Portuguese freshwaters, with C-phycocyanin and chlorophylls, compounds with a high market value, being abundantly produced by the species. This work presents the development of a sequential downstream process to obtain C-phycocyanin and chlorophyll from A. cylindrica. The C-phycocyanin extraction is carried using Na-phosphate (20 mM, pH 7) buffer, while aqueous solutions of surface-active compounds (250 mM), as well as ethanol, were screened for the extraction of the chlorophylls. After selecting the best solvents to recover both pigments from A. cylindrica , the operational conditions (solid-liquid ratio, time of extraction and temperature) were optimized, allowing the recovery of around 90% and 55% of the total content of C-phycocyanin and chlorophylls, respectively. Finally, dextran/ copolymer-based ABS were applied to promote the purification of C-phycocyanin from the residual chlorophylls and other contaminant proteins, leading to an increase in the C-phycocyanin purity of 4-fold, without compromising its photostability.
publisher	ELSEVIER
issn	1383-5866
isbn	1873-3794
year published	2021
volume	255
digital object identifier (doi)	10.1016/j.seppur.2020.117538
web of science category	9
subject category	Engineering, Chemical
unique article identifier	WOS:000633042200004