authors |
Sintra, TE; Bagagem, SS; Ahsaie, FG; Fernandes, A; Martins, M; Macario, IPE; Pereira, JL; Goncalves, FJM; Pazuki, G; Coutinho, JAP; Ventura, SPM |
nationality |
International |
journal |
SEPARATION AND PURIFICATION TECHNOLOGY |
author keywords |
Cyanobacteria; Anabaena cylindrica; C-phycocyanin; Chlorophylls; Sequential extraction; Purification; Aqueous biphasic systems; Stability |
keywords |
AQUEOUS 2-PHASE SYSTEMS; IONIC-LIQUID; MEDIATED EXTRACTION; BIPHASIC SYSTEMS; EFFICIENT METHOD; BLOCK-COPOLYMER; DRY BIOMASS; PURIFICATION; SEPARATION; OPTIMIZATION |
abstract |
Cyanobacteria are attracting the attention worldwide as a reliable and sustainable feedstock for the production of biofuels, food colorants and biochemically active compounds. Anabaena cylindrica (A. cylindrica) is a cyanobacterium with a significant widespread occurrence in Portuguese freshwaters, with C-phycocyanin and chlorophylls, compounds with a high market value, being abundantly produced by the species. This work presents the development of a sequential downstream process to obtain C-phycocyanin and chlorophyll from A. cylindrica. The C-phycocyanin extraction is carried using Na-phosphate (20 mM, pH 7) buffer, while aqueous solutions of surface-active compounds (250 mM), as well as ethanol, were screened for the extraction of the chlorophylls. After selecting the best solvents to recover both pigments from A. cylindrica , the operational conditions (solid-liquid ratio, time of extraction and temperature) were optimized, allowing the recovery of around 90% and 55% of the total content of C-phycocyanin and chlorophylls, respectively. Finally, dextran/ copolymer-based ABS were applied to promote the purification of C-phycocyanin from the residual chlorophylls and other contaminant proteins, leading to an increase in the C-phycocyanin purity of 4-fold, without compromising its photostability. |
publisher |
ELSEVIER |
issn |
1383-5866 |
isbn |
1873-3794 |
year published |
2021 |
volume |
255 |
digital object identifier (doi) |
10.1016/j.seppur.2020.117538 |
web of science category |
9 |
subject category |
Engineering, Chemical |
unique article identifier |
WOS:000633042200004
|
ciceco authors
impact metrics
journal analysis (jcr 2019):
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journal impact factor |
5.774 |
5 year journal impact factor |
5.257 |
category normalized journal impact factor percentile |
89.161 |
dimensions (citation analysis):
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altmetrics (social interaction):
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